Novel Biopolymer Matrices for Microencapsulation of Phages: Enhanced Protection Against Acidity and Protease Activity

被引：73

作者：

Dini, Cecilia ^{[1
,2
]}

Islan, German A. ^{[3
]}

de Urraza, Patricio J. ^{[1
,2
]}

Castro, Guillermo R. ^{[3
]}

机构：

[1] UNLP, Catedra Microbiol, Fac Ciencias Exactas, RA-1900 La Plata, Buenos Aires, Argentina

[2] CIDCA CONICET UNLP CCT La Plata, RA-1900 La Plata, Buenos Aires, Argentina

[3] CINDEFI CONICET UNLP CCT La Plata, RA-1900 La Plata, Buenos Aires, Argentina

来源：

MACROMOLECULAR BIOSCIENCE | 2012年 / 12卷 / 09期

关键词：

biocontrol; biological applications of polymers; biopolymers; microencapsulation; phages; ESCHERICHIA-COLI O157-H7; HEMOLYTIC-UREMIC SYNDROME; BACTERIOPHAGES; HYDROGELS; CATTLE; PROTEINS; CHITOSAN; BEHAVIOR; GELATION; RELEASE;

D O I：

10.1002/mabi.201200109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phage therapy by oral administration requires enhanced resistance of phages to the harsh gastric conditions. The aim of this work is the microencapsulation of phages in natural biopolymeric matrices as a protective barrier against the gastric environment. Alginate and pectin are used as base polymers. Further emulsification with oleic acid or coating with a different biopolymer is also studied. Emulsified pectin shows the maximum encapsulation efficiency and the highest protection against acidity, leaving more than 103 active phages after 30?min exposure at pH?=?1.6, and protects phage from pepsin activity (4.2?mg?mL-1). Non-encapsulated phages are fully inactivated at pH?=?1.6 or with pepsin (0.5?mg?mL-1) after 10?min.

引用

页码：1200 / 1208

页数：9

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