Calcyclin binding protein promotes DNA synthesis and differentiation in rat neonatal cardiomyocytes

被引：32
作者：
Au, Ka-Wing
Kou, Cecy Y. C.
Woo, Anthony Y. H.
Chim, Stephen S. C.
Fung, Kwok-Pui
Cheng, Christopher H. K.
Waye, Mary M. Y.
Tsui, Stephen K. W.
机构：
[1] Chinese Univ Hong Kong, Dept Biochem, Shatin, Hong Kong, Peoples R China
[2] Chinese Univ Hong Kong, Croucher Lab Human Genom, Shatin, Hong Kong, Peoples R China
来源：
JOURNAL OF CELLULAR BIOCHEMISTRY | 2006年 / 98卷 / 03期
关键词：
CacyBP/SIP; DNA synthesis; differentiation; H9C2; cardiomyocytes;
D O I：
10.1002/jcb.20710
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
During cardiac muscle devolopment, most cardiomyocytes permanently withdraw from the cell cycle. Previously, by suppressive subtractive hybridization, we identified calcylclin-binding protein/Siah-interacting protein important role of CacyBP/SIP in cardiac devolopment. To show the importance of CacyBP/SIP during myoblast differentiation, we report here that cacyBP/SIP is devolopmentally regulated in postnatal rat hearts. The overexpression of CacyBp/SIP promotes the differentiation and DNA synthesis of H9C2 cells and primary rat cardiomyocytes, as well as downregulates the expression of beta-catenin. Besides, CacyBP/SIP promotes the formation of myotubes and multinucleation upon differentiation. To investigate the cardioprotective role of CacyBP/SIP in cardiomyocytes, a hypoxia/reoxygenation model was employed. We found that CacyBP/SIP was upregulated during myocardial infarction (MI) and hypoxia/reoxygenation. As a conclusion, CacyBP/SIP may play a role in cardiomyogenic differentiation and possibly protection of cardiomyocytes during hypoxia/reoxygenation injury.
引用
收藏
页码：555 / 566
页数：12
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