Resistance of different Chlamydia-like organisms to quinolones and mutations in the quinoline resistance-determining region of the DNA gyrase A- and topoisomerase-encoding genes

被引：26
作者：
Casson, Nicola [1 ]
Greub, Gilbert [1 ]
机构：
[1] Univ Lausanne, Fac Biol & Med, Inst Microbiol, Ctr Res Intracellular Bacteria, CH-1011 Lausanne, Switzerland
来源：
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | 2006年 / 27卷 / 06期
关键词：
QRDR; DNA gyrase; topoisomerase; quinolones; Chlamydia; Chlamydia-like organisms;
D O I：
10.1016/j.ijantimicag.2006.03.009
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Parachlamydia acanthamoebae has been shown to be resistant to fluoroquinolones. In this study, we determined the susceptibility of Neochlamydia hartmannellae and Simkania negevensis to quinolones using amoebal co-culture in the presence of serial dilutions of ciprofloxacin. The gyrA and parC quinoline resistance-determining regions (QRDRs) were amplified and sequenced, and amino acid substitutions that may explain the observed quinolone resistance were investigated. Neochlamydia and Simkania were resistant to 16 mu g/mL ciprofloxacin. Substitution at positions 83 and 70 of GyrA QRDR were present in Neochlamydia and in both Parachlamydia strains studied. For Simkania, substitutions at positions 99 and 10 1 in the GyrA QRDR and at positions 81 and 84 in the ParC QRDR were observed. Resistance of Parachlamydia, Neochlamydia and Simkania to quinolones is likely due to these Mutations. (c) 2006 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
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收藏
页码：541 / 544
页数：4
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