Efficacy of an Adjuvanted Middle East Respiratory Syndrome Coronavirus Spike Protein Vaccine in Dromedary Camels and Alpacas

被引：72

作者：

Adney, Danielle R. ^{[1
]}

Wang, Lingshu ^{[2
]}

van Doremalen, Neeltje ^{[3
]}

Shi, Wei ^{[2
]}

Zhang, Yi ^{[2
]}

Kong, Wing-Pui ^{[2
]}

Miller, Megan R. ^{[3
]}

Bushmaker, Trenton ^{[3
]}

Scott, Dana ^{[3
]}

de Wit, Emmie ^{[3
]}

Modjarrad, Kayvon ^{[4
]}

Petrovsky, Nikolai ^{[5
,6
]}

Graham, Barney S. ^{[2
]}

Bowen, Richard A. ^{[1
]}

Munster, Vincent J. ^{[1
,3
]}

机构：

[1] Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80521 USA

[2] NIAID, Vaccine Res Ctr, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA

[3] NIAID, Lab Virol, Div Intramural Res, NIH, Hamilton, MT 59840 USA

[4] Walter Reed Army Inst Res, Mil HIV Res, Silver Spring, MD 20910 USA

[5] Flinders Univ S Australia, Bedford Pk, SA 5042, Australia

[6] Flinders Med Ctr, Vaxine Pty Ltd, Bedford, SA 5042, Australia

来源：

VIRUSES-BASEL | 2019年 / 11卷 / 03期

基金：

美国国家卫生研究院;

关键词：

MERS-CoV; camels; vaccines; One Health; MERS-COV INFECTION; TO-HUMAN TRANSMISSION; HIGH PREVALENCE; SAUDI-ARABIA; ANTIBODIES; REPLICATION; EXPOSURE; RISK;

D O I：

10.3390/v11030212

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

MERS-CoV is present in dromedary camels throughout the Middle East and Africa. Dromedary camels are the primary zoonotic reservoir for human infections. Interruption of the zoonotic transmission chain from camels to humans, therefore, may be an effective strategy to control the ongoing MERS-CoV outbreak. Here we show that vaccination with an adjuvanted MERS-CoV Spike protein subunit vaccine confers complete protection from MERS-CoV disease in alpaca and results in reduced and delayed viral shedding in the upper airways of dromedary camels. Protection in alpaca correlates with high serum neutralizing antibody titers. Lower titers of serum neutralizing antibodies correlate with delayed and significantly reduced shedding in the nasal turbinates of dromedary camels. Together, these data indicate that induction of robust neutralizing humoral immune responses by vaccination of naive animals reduces shedding that potentially could diminish the risk of zoonotic transmission.

引用

页数：13

共 36 条

[1] Adney DR, 2016, EMERG INFECT DIS, V22, P1031, DOI [10.3201/eid2206.160192, 10.3201/2206.160192]
[2] Replication and Shedding of MERS-CoV in Upper Respiratory Tract of Inoculated Dromedary Camels
Adney, Danielle R.
van Doremalen, Neeltje
Brown, Vienna R.
Bushmaker, Trenton
Scott, Dana
de Wit, Emmie
Bowen, Richard A.
Munster, Vincent J.
[J]. EMERGING INFECTIOUS DISEASES, 2014, 20 (12) : 1999 - 2005
[3] Asymptomatic MERS-CoV Infection in Humans Possibly Linked to Infected Dromedaries Imported from Oman to United Arab Emirates, May 2015
Al Hammadi, Zulaikha M.
Chu, Daniel K. W.
Eltahir, Yassir M.
Al Hosani, Farida
Al Mulla, Mariam
Tarnini, Wasim
Hall, Aron J.
Perera, Ranawaka A. P. M.
Abdelkhalek, Mohamed M.
Peiris, J. S. M.
Al Muhairi, Salama S.
Poon, Leo L. M.
[J]. EMERGING INFECTIOUS DISEASES, 2015, 21 (12) : 2197 - 2200
[4] Middle East Respiratory Syndrome Coronavirus Infection in Dromedary Camels in Saudi Arabia
Alagaili, Abdulaziz N.
Briese, Thomas
Mishra, Nischay
Kapoor, Vishal
Sameroff, Stephen C.
Burbelo, Peter D.
de Wit, Emmie
Munster, Vincent J.
Hensley, Lisa E.
Zalmout, Iyad S.
Kapoor, Amit
Epstein, Jonathan H.
Karesh, William B.
Daszak, Peter
Mohammed, Osama B.
Lipkin, W. Ian
[J]. MBIO, 2014, 5 (02):
[5] Detection of the Middle East Respiratory Syndrome Coronavirus Genome in an Air Sample Originating from a Camel Barn Owned by an Infected Patient
Azhar, Esam I.
Hashem, Anwar M.
El-Kafrawy, Sherif A.
Sohrab, Sayed Sartaj
Aburizaiza, Asad S.
Farraj, Suha A.
Hassan, Ahmed M.
Al-Saeed, Muneera S.
Jamjoom, Ghazi A.
Madani, Tariq A.
[J]. MBIO, 2014, 5 (04):
[6] Evidence for Camel-to-Human Transmission of MERS Coronavirus
Azhar, Esam I.
El-Kafrawy, Sherif A.
Farraj, Suha A.
Hassan, Ahmed M.
Al-Saeed, Muneera S.
Hashem, Anwar M.
Madani, Tariq A.
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 2014, 370 (26) : 2499 - 2505
[7] Unraveling the drivers of MERS-CoV transmission
Cauchemez, Simon
Nouvellet, Pierre
Cori, Anne
Jombart, Thibaut
Garske, Tini
Clapham, Hannah
Moore, Sean
Mills, Harriet Linden
Salje, Henrik
Collins, Caitlin
Rodriquez-Barraquer, Isabel
Riley, Steven
Truelove, Shaun
Algarni, Homoud
Alhakeem, Rafat
AlHarbi, Khalid
Turkistani, Abdulhafiz
Aguas, Ricardo J.
Cummings, Derek A. T.
Van Kerkhove, Maria D.
Donnelly, Christl A.
Lessler, Justin
Fraser, Christophe
Al-Barrak, Ali
Ferguson, Neil M.
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (32) : 9081 - 9086
[8] MERS Coronaviruses in Dromedary Camels, Egypt
Chu, Daniel K. W.
Poon, Leo L. M.
Gomaa, Mokhtar M.
Shehata, Mahmoud M.
Perera, Ranawaka A. P. M.
Abu Zeid, Dina
El Rifay, Amira S.
Siu, Lewis Y.
Guan, Yi
Webby, Richard J.
Ali, Mohamed A.
Peiris, Malik
Kayali, Ghazi
[J]. EMERGING INFECTIOUS DISEASES, 2014, 20 (06) : 1049 - 1053
[9] Antibodies against MERS Coronavirus in Dromedary Camels, Kenya, 1992-2013
Corman, Victor M.
Jores, Joerg
Meyer, Benjamin
Younan, Mario
Liljander, Anne
Said, Mohammed Y.
Gluecks, Ilona
Lattwein, Erik
Bosch, Berend-Jan
Drexler, Jan Felix
Bornstein, Set
Drosten, Christian
Mueller, Marcel A.
[J]. EMERGING INFECTIOUS DISEASES, 2014, 20 (08) : 1319 - 1322
[10] Experimental Infection and Response to Rechallenge of Alpacas with Middle East Respiratory Syndrome Coronavirus
Crameri, Gary
Durr, Peter A.
Klein, Reuben
Foord, Adam
Yu, Meng
Riddell, Sarah
Haining, Jessica
Johnson, Dayna
Hemida, Maged G.
Barr, Jennifer
Peiris, Malik
Middleton, Deborah
Wang, Lin-Fa
[J]. EMERGING INFECTIOUS DISEASES, 2016, 22 (06) : 1071 - 1074

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