Development and Evaluation of PCR Assay Based on Outer Membrane Protein 22 Gene for Genus Specific Diagnosis of Brucella spp

被引：3

作者：

Minhas, Priyanka ^{[1
]}

Ramneek ^{[1
]}

Pawar, Hitesh N. ^{[1
]}

Kaur, Daljit ^{[1
]}

Deka, Dipak ^{[1
]}

Agrawal, Ravi Kant ^{[1
]}

机构：

[1] Guru Angad Dev Vet & Anim Sci Univ, Sch Anim Biotechnol, Ludhiana 141004, Punjab, India

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES INDIA SECTION B-BIOLOGICAL SCIENCES | 2013年 / 83卷 / 04期

关键词：

Brucellosis; Brucella; Diagnosis; Polymerase chain reaction; Outer membrane protein 22; DNA AMPLIFICATION; VACCINE STRAINS; B; ABORTUS; IDENTIFICATION; MELITENSIS; DIFFERENTIATION; MILK; SUIS;

D O I：

10.1007/s40011-013-0174-x

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Brucellosis is a worldwide zoonosis and a significant cause of loss of health in human and animals. Conventionally, definitive diagnosis is done by isolation of brucellae which is time consuming, technically demanding and may be hazardous. The present study was aimed to develop PCR assay based on evolutionarily conserved outer membrane protein 22 gene for rapid diagnosis of Brucella spp. A set of primer was designed after multiple alignment of outer membrane protein 22 gene sequences by clustalW. PCR amplification resulted in single specific amplicon of 639 bp in all the Brucella strains tested. The primers were found to be highly sensitive and specific. PCR assay developed may be used for clinical diagnosis of Brucella infection.

引用

页码：615 / 619

页数：5

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