Selection and characterization of colorectal cancer cell-specific peptides

被引：10

作者：

Wang, Han ^{[1
]}

Ma, Caixia ^{[1
]}

Li, Rui ^{[1
]}

Guo, Yonge ^{[1
]}

He, Yakun ^{[1
]}

Wang, Xiaojing ^{[1
]}

Chen, Yiwen ^{[1
]}

Hou, Yingchun ^{[1
]}

机构：

[1] Shaanxi Normal Univ, Dept Cell Biol, Coll Life Sci, Xian 710062, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2013年 / 35卷 / 05期

关键词：

Colorectal cancer; Molecular imaging; Peptide probe; Phage display; PHAGE DISPLAY SELECTION; TUMOR VASCULATURE; BINDING PEPTIDE; DRUG-DELIVERY; IDENTIFICATION; PROBE;

D O I：

10.1007/s10529-013-1145-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To develop a novel peptide probe for imaging detection of colorectal cancer, a 12-mer phage display library was used to select peptides that bind specifically to the human colorectal cancer cell line Caco-2. After four rounds of panning, four phage clones that bound specifically to the Caco-2 cells were selected. The phage clone SP-2 had a particularly high affinity and specificity for Caco-2 cells. This clone was identified using a series of methods. The peptide SP-2 that was displayed on phage SP-2 exhibited a high specificity to Caco-2 cells. Thus, the peptide SP-2 could be a candidate probe for the detection of colorectal cancer.

引用

页码：671 / 677

页数：7

共 11 条

[1] Evaluation of 64Cu Labeled GX1: A Phage Display Peptide Probe for PET Imaging of Tumor Vasculature [J].

Chen, Kai ;

Sun, Xilin ;

Niu, Gang ;

Ma, Ying ;

Yap, Li-Peng ;

Hui, Xiaoli ;

Wu, Kaichun ;

Fan, Daiming ;

Conti, Peter S. ;

Chen, Xiaoyuan .

MOLECULAR IMAGING AND BIOLOGY, 2012, 14 (01) :96-105

[2] Phage Display in Molecular Imaging and Diagnosis of Cancer [J].

Deutscher, Susan L. .

CHEMICAL REVIEWS, 2010, 110 (05) :3196-3211

[3] 111In-labeled KCCYSL peptide as an imaging probe for ErbB-2-expressing ovarian carcinomas [J].

Deutscher, Susan L. ;

Figueroa, Said D. ;

Kumar, Senthil R. .

JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS, 2009, 52 (13-14) :583-590

[4] Targeted drug delivery to tumor vasculature by a carbohydrate mimetic peptide [J].

Hatakeyama, Shingo ;

Sugihara, Kazuhiro ;

Shibata, Toshiaki K. ;

Nakayama, Jun ;

Akama, Tomoya O. ;

Tamura, Naoaki ;

Wong, Shuk-Man ;

Bobkov, Andrey A. ;

Takano, Yutaka ;

Ohyama, Chikara ;

Fukuda, Minoru ;

Fukuda, Michiko N. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (49) :19587-19592

[5] Isolation of peptide ligands that interact specifically with human glioma cells [J].

Ho, Ivy A. W. ;

Hui, Kam M. ;

Lam, Paula Y. P. .

PEPTIDES, 2010, 31 (04) :644-650

[6] Stage-specific vascular markers revealed by phage display in a mouse model of pancreatic islet tumorigenesis [J].

Joyce, JA ;

Laakkonen, P ;

Bernasconi, M ;

Bergers, G ;

Ruoslahti, E ;

Hanahan, D .

CANCER CELL, 2003, 4 (05) :393-403

[7] Isolation of a colon tumor specific binding peptide using phage display selection [J].

Kelly, KA ;

Jones, DA .

NEOPLASIA, 2003, 5 (05) :437-444

[8] A novel peptide specifically targeting the vasculature of orthotopic colorectal cancer for imaging detection and drug delivery [J].

Li, Zhi Jie ;

Wu, William Ka Kei ;

Ng, Simon Siu Man ;

Yu, Le ;

Li, Hai Tao ;

Wong, Clover Ching Man ;

Wu, Ya Chun ;

Zhang, Lin ;

Ren, Shun Xiang ;

Sun, Xue Gang ;

Chan, Kam Ming ;

Cho, Chi Hin .

JOURNAL OF CONTROLLED RELEASE, 2010, 148 (03) :292-302

[9] Selection of silk-binding peptides by phage display [J].

Nomura, Yoko ;

Sharma, Vandana ;

Yamamura, Asami ;

Yokobayashi, Yohei .

BIOTECHNOLOGY LETTERS, 2011, 33 (05) :1069-1073

[10] Identification of nose-to-brain homing peptide through phage display [J].

Wan, Xiao-Mei ;

Chen, Yong-Ping ;

Xu, Wen-Rui ;

Yang, Wen-jun ;

Wen, Long-Ping .

PEPTIDES, 2009, 30 (02) :343-350

← 1 2 →