IL-17A but not IL-22 suppresses the replication of hepatitis B virus mediated by over-expression of MxA and OAS mRNA in the HepG2.2.15 cell line

被引:43
作者
Wang, Bing [1 ,2 ]
Zhao, Xin-Ping [3 ]
Fan, Yu-Chen [1 ,2 ]
Zhang, Jian-Jun [1 ]
Zhao, Jing [1 ]
Wang, Kai [1 ,2 ]
机构
[1] Shandong Univ, Qilu Hosp, Dept Hepatol, Jinan 250012, Peoples R China
[2] Shandong Univ, Inst Hepatol, Jinan 250012, Peoples R China
[3] Third Peoples Hosp Zaozhuang, Zaozhuang 277100, Peoples R China
基金
中国国家自然科学基金;
关键词
Interleukin-17A; Interleukin-22; Hepatitis B virus; Myxovirus resistance A; Oligoadenylate synthetase; DISEASE PROGRESSION; INTERFERON; LIVER; INTERLEUKIN-22; INHIBITION; DNA; HEPATOCYTES; ACTIVATION; THERAPY; PROTEIN;
D O I
10.1016/j.antiviral.2012.12.018
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Interleukin-17A (IL-17A) and interleukin-22 (IL-22), mainly secreted by interleukin-17-producing T help cells (Th17), are pleiotropic cytokines that regulate the biological responses of several target cells, including hepatocytes. Th17 frequency was reported to negatively correlate with plasma hepatitis B virus (HBV) DNA load in patients with HBV infection. Several studies have indicated that cytokines, such as IL-6 and IL-4, are involved in the noncytopathic suppression of HBV replication. We therefore hypothesized that IL-17A and IL-22 might have a potent suppressive effect on HBV replication. In our present study, we analyzed the suppressive effect of IL-17A and IL-22 on HBV replication in the hepatocellular carcinoma cell line HepG2.2.15. IL-17A did not inhibit the proliferation of HepG2.2.15 cells. It decreased the levels of HBV s antigen (HBsAg) and HBV e antigen (HBeAg) in culture medium and the levels of intracellular HBV DNA. By contrast, blockage of IL-17 receptor (IL-17R) increased the levels of HBsAg and extracellular HBV DNA in culture medium and the levels of intracellular HBV DNA. The expression of antiviral proteins, including myxovirus resistance A (MxA) and oligoadenylate synthetase (OAS), was enhanced by IL-17A. IL-22 and anti-human IL-22 receptor (IL-22R) antibody did not change any indexes. We demonstrated that IL-17A effectively suppressed HBV replication in a noncytopathic manner and the over-expression of MxA and OAS mRNA was involved in the suppression of HBV replication by IL-17A. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:285 / 292
页数:8
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