A Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) Method for the Determination of Free Hydroxy Fatty Acids in Cow and Goat Milk

被引：25

作者：

Kokotou, Maroula G. ^{[1
]}

Mantzourani, Christiana ^{[1
]}

Bourboula, Asimina ^{[1
]}

Mountanea, Olga G. ^{[1
]}

Kokotos, George ^{[1
]}

机构：

[1] Natl & Kapodistrian Univ Athens, Dept Chem, Athens 15771, Greece

来源：

MOLECULES | 2020年 / 25卷 / 17期

关键词：

determination; hydroxy fatty acids; high resolution mass spectrometry; liquid chromatography; milk; LIPIDS; ESTERS;

D O I：

10.3390/molecules25173947

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A liquid chromatography-high resolution mass spectrometry (LC-HRMS) method for the direct determination of various saturated hydroxy fatty acids (HFAs) in milk was developed for the first time. The method involves mild sample preparation conditions, avoids time-consuming derivatization procedures, and permits the simultaneous determination of 19 free HFAs in a single 10-min run. This method was validated and applied in 17 cow milk and 12 goat milk samples. This work revealed the existence of various previously unrecognized hydroxylated positional isomers of palmitic acid and stearic acid in both cow and goat milk, expanding our knowledge on the lipidome of milk. The most abundant free HFAs in cow milk were proven to be 7-hydroxystearic acid (7HSA) and 10-hydroxystearic acid (10HSA) (mean content values of 175.1 +/- 3.4 mu g/mL and 72.4 +/- 6.1 mu g/mL in fresh milk, respectively). The contents of 7HSA in cow milk seem to be substantially higher than those in goat milk.

引用

页数：12

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