UVB Irradiation Induced Cell Damage and Early Onset of Junbb Expression in Zebrafish

被引:6
作者
Chen, Rui-Yi [1 ,2 ]
Lin, Chun-Ju [3 ]
Liang, Sung-Tzu [3 ]
Villalobos, Omar [4 ]
Villaflores, Oliver B. [5 ]
Lou, Bao [6 ]
Lai, Yu-Heng [7 ]
Hsiao, Chung-Der [3 ,8 ,9 ]
机构
[1] Marine Fisheries Res Inst Zhejiang, Key Lab Mariculture & Enhancement Zhejiang Prov, Zhoushan 316100, Peoples R China
[2] Zhejiang Ocean Univ, Marine & Fishery Inst, Zhoushan 316100, Peoples R China
[3] Chung Yuan Christian Univ, Dept Biosci Technol, Chungli 32023, Taiwan
[4] Univ Santo Tomas, Fac Pharm, Dept Pharm, Manila 1015, Philippines
[5] Univ Santo Tomas, Fac Pharm, Dept Biochem, Manila 1013, Philippines
[6] Zhejiang Acad Agr Sci, Inst Hydrobiol, Shiqiao Rd 198, Hangzhou 310021, Peoples R China
[7] Chinese Culture Univ, Dept Chem, Taipei 11114, Taiwan
[8] Chung Yuan Christian Univ, Dept Chem, Chungli 32023, Taiwan
[9] Chung Yuan Christian Univ, Ctr Nanotechnol, Chungli 32023, Taiwan
来源
ANIMALS | 2020年 / 10卷 / 06期
关键词
UVB; zebrafish; skin; biomarker; microarray; PITUITARY-ADRENAL AXIS; INDUCED DNA-DAMAGE; ULTRAVIOLET-RADIATION; B RADIATION; SIGNALING PATHWAY; IN-VIVO; SKIN; KERATINOCYTES; APOPTOSIS; MELANOMA;
D O I
10.3390/ani10061096
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Simple Summary Zebrafish is a good in vivo model to study how skin responds to Ultraviolet B (UVB) irradiation at the cellular, molecular, and whole organism levels. Previous studies showed that zebrafish embryo fin undergoes extensive shrinkage after exposure to UVB irradiation, and this phenotypic change can be assessed using antioxidant drugs. To provide more detailed chronological changes for zebrafish embryos after receiving UVB irradiation, sequential alterations of zebrafish embryos at morphological (fin), cellular (cell death, oxidative stress, immune-response, and marker gene expression) and molecular (microarray screen and real-time RT-PCR assay) levels were examined in this study. The results showed thatjunbbgene expression was activated as early as 3 h post-UVB irradiation, followed by significant elevation of apoptosis around 9 h post-UVB irradiation, neutrophil migration to the wound area approximately 14 h post-UVB irradiation, and activation ofmmpgene expression at around 24 h post-UVB irradiation. These chronological cellular and molecular responses after UVB irradiation in zebrafish provide a basic and fundamental foundation for future line construction and UVB-associated gene validation. Ultraviolet B (UVB) radiation has drawn more attention over these past few decades since it causes severe DNA damage and induces inflammatory response. Serial gene profiling and high throughput data in UVB-associated phenomenon in human cultured cells or full rack of human skin have been investigated. However, results using different tissue models lead to ambiguity in UVB-induced pathways. In order to systematically understand the UVB-associated reactions, the zebrafish model was used, and whole organism gene profiling was performed to identify a novel biomarker which can be used to generate a new mechanistic approach for further screening on a UVB-related system biology. In this study, detailed morphological assays were performed to address biological response after receiving UVB irradiation at morphological, cellular, and molecular levels. Microarray screening and whole genome profiling revealed that there is an early onset expression ofjunbbin zebrafish embryos after UVB irradiation. Also, the identified novel biomarkerjunbbis more sensitive to UVB response thanmmpswhich have been used in mouse models. Moreover, cellular and molecular response chronology after UVB irradiation in zebrafish provide a solid and fundamental mechanism for use in a UV radiation-associated study in the future.
引用
收藏
页码:1 / 16
页数:16
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