Isotopic labeling-assisted metabolomics using LC-MS

被引：73

作者：

Bueschl, C. ^{[1
]}

Krska, R. ^{[1
]}

Kluger, B. ^{[1
]}

Schuhmacher, R. ^{[1
]}

机构：

[1] Univ Nat Resources & Life Sci, Dept Agrobiotechnol IFA Tulln, Ctr Analyt Chem, A-3430 Vienna, Tulln, Austria

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2013年 / 405卷 / 01期

基金：

奥地利科学基金会;

关键词：

Bioanalytical methods; Mass spectrometry; Metabolomics; Liquid chromatography; CHROMATOGRAPHY-MASS SPECTROMETRY; RELATIVE QUANTIFICATION; DATA SETS; EXTRACTION; ELUCIDATION; ANNOTATION; METABOLISM; MYCOTOXINS; PROTEOMICS; ABSOLUTE;

D O I：

10.1007/s00216-012-6375-y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Metabolomics has emerged as the latest of the so-called "omics" disciplines and has great potential to provide deeper understanding of fundamental biochemical processes at the biological system level. Among recent technological developments, LC-HRMS enables determination of hundreds to thousands of metabolites over a wide range of concentrations and has developed into one of the most powerful techniques in non-targeted metabolomics. The analysis of mixtures of in-vivo-stable isotopic-labeled samples or reference substances with un-labeled samples leads to specific LC-MS data patterns which can be systematically exploited in practically all data-processing steps. This includes recognition of true metabolite-derived analytical features in highly complex LC-MS data and characterization of the global biochemical composition of biological samples. In addition, stable-isotopic labeling can be used for more accurate quantification (via internal standardization) and identification of compounds in different organisms.

引用

页码：27 / 33

页数：7

共 36 条

[1] The ion suppression phenomenon in liquid chromatography-mass spectrometry and its consequences in the field of residue [J].

Antignac, JP ;

de Wasch, K ;

Monteau, F ;

De Brabander, H ;

Andre, F ;

Le Bizec, B .

ANALYTICA CHIMICA ACTA, 2005, 529 (1-2) :129-136

[2] Isotopic enrichment enhancement in metabonomic analysis of UPLC-MS data sets [J].

Athersuch, Toby J. ;

Nicholson, Jeremy K. ;

Wilson, Ian D. .

JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS, 2007, 50 (5-6) :303-307

[3]

Beecher, 2010, U.S. patent, Patent No. [7,820,963, 7820963]

[4] Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach [J].

Bennett, Bryson D. ;

Yuan, Jie ;

Kimball, Elizabeth H. ;

Rabinowitz, Joshua D. .

NATURE PROTOCOLS, 2008, 3 (08) :1299-1311

[5] Chromatographic methods for the simultaneous determination of mycotoxins and their conjugates in cereals [J].

Berthiller, Franz ;

Sulyok, Michael ;

Krska, Rudolf ;

Schuhmacher, Rainer .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2007, 119 (1-2) :33-37

[6] Metabolic labeling of proteins for proteomics [J].

Beynon, RJ ;

Pratt, JM .

MOLECULAR & CELLULAR PROTEOMICS, 2005, 4 (07) :857-872

[7] Metabolome analysis:: the potential of in vivo labeling with stable isotopes for metabolite profiling [J].

Birkemeyer, C ;

Luedemann, A ;

Wagner, C ;

Erban, A ;

Kopka, J .

TRENDS IN BIOTECHNOLOGY, 2005, 23 (01) :28-33

[8] MetExtract: a new software tool for the automated comprehensive extraction of metabolite-derived LC/MS signals in metabolomics research [J].

Bueschl, Christoph ;

Kluger, Bernhard ;

Berthiller, Franz ;

Lirk, Gerald ;

Winkler, Stephan ;

Krska, Rudolf ;

Schuhmacher, Rainer .

BIOINFORMATICS, 2012, 28 (05) :736-738

[9] LC-MS-based metabolomics in drug metabolism [J].

Chen, Chi ;

Gonzalez, Frank J. ;

Idle, Jeffrey R. .

DRUG METABOLISM REVIEWS, 2007, 39 (2-3) :581-597

[10] Metabolomics - the link between genotypes and phenotypes [J].

Fiehn, O .

PLANT MOLECULAR BIOLOGY, 2002, 48 (1-2) :155-171

← 1 2 3 4 →