Stability-Indicating LC Method for Assay of Cholecalciferol

被引：5
|
作者：
Desai, Namita D. [1 ]
Singh, Pirthipal P. [1 ]
Amin, Purnima D. [1 ]
Jain, Satishkumar P. [1 ]
机构：
[1] Univ Mumbai, Univ Inst Chem Technol Autonomous, Pharmaceut Sci & Technol Div, Bombay 400019, Maharashtra, India
来源：
CHROMATOGRAPHIA | 2009年 / 69卷 / 3-4期
关键词：
Column liquid chromatography; Cholecalciferol; Stability-indicating;
D O I：
10.1365/s10337-008-0914-x
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This paper discusses the development of a stability-indicating reversed-phase LC method for analysis of cholecalciferol as the bulk drug and in formulations. The mobile phase was acetonitrile-methanol-water 50:50:2 (v/v). The calibration plot for the drug was linear in the range 0.4-10 mu g mL(-1). The method was accurate and precise with limits of detection and quantitation of 64 and 215 ng, respectively. Mean recovery was 100.71%. The method was used for analysis of cholecalciferol in pharmaceutical formulations in the presence of its degradation products and commonly used excipients.
引用
收藏
页码：385 / 388
页数：4
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