Molecular characterization and expression analysis of interleukin-15 (IL-15) genes in orange-spotted grouper (Epinephelus coioides) in response to Vibrio harveyi challenge

被引：2
|
作者：
Wu, Fan [1 ,2 ,3 ,4 ]
Wang, Zhiwen [1 ,2 ,3 ,4 ]
Yang, Guanjian [1 ,2 ,3 ,4 ]
Jian, Jichang [3 ,4 ]
Lu, Yishan [1 ,2 ,3 ,4 ]
机构：
[1] Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518120, Peoples R China
[2] Guangdong Prov Engn Res Ctr Aquat Anim Hlth Assess, Shenzhen 51820, Peoples R China
[3] Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524025, Peoples R China
[4] Guangdong Prov Key Lab Aqua Anim Dis Control & Hlt, Zhanjiang 524025, Peoples R China
来源：
FISH & SHELLFISH IMMUNOLOGY | 2022年 / 128卷
关键词：
IL15; Orange-spotted grouper; Vibrio harveyi; T-CELLS; INTERFERON-GAMMA; HUMAN MONOCYTES; MESSENGER-RNA; CLONING; ALPHA; IDENTIFICATION; HOMEOSTASIS; (IL)-15; BIOLOGY;
D O I：
10.1016/j.fsi.2022.08.003
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
As a member of the gamma c family, interleukin 15 plays an important function in the immune response. In this study, we cloned an IL15 from Epinephelus coioides (named Ec-IL15). The open reading frame of Ec-IL15 is 528 bp, encoding 175 amino acids. Sequence alignment analysis showed that EcIL-15 has a conserved Pfam: IL15 domain and four cysteine residues. Subcellular localization studies have shown that Ec-IL15 is distributed in whole cells. In healthy groupers, Ec-IL15 was expressed in all 11 tissues tested and the highest in liver. After ConA, PHA, LPS and poly I:C stimulation, Ec-IL15 expression of HKLs was significantly upregulated. After V. harveyi infection, the expression of Ec-IL15 in 9 tissues was significantly upregulated and peaked within 48 h. In addition, recombinant Ec-IL15 protein can not only stimulate HKLs proliferation and cytokine expression, but also has the potential as an immune enhancer.
引用
收藏
页码：327 / 334
页数：8
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