Luteolibacter luteussp. nov., isolated from stream bank soil

A non-motile, Gram-stain-negative, rod-shaped and yellow-colored bacterium, designated G-1-1-1(T)was obtained from soil sampled at Gwanggyo stream bank, Gyeonggi-do, Republic of Korea. Cells were aerobic, catalase positive, grew optimally at 25-30 degrees C and hydrolysed aesculin and casein. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain G-1-1-1(T)formed a lineage within the genusLuteolibacter. The closest members wereLuteolibacter flavescensGKX(T)(97.7% sequence similarity) andLuteolibacter arcticusMC 3726(T)(97.3%). The sequence similarities with other members of the genusLuteolibacterwere <= 93.9%. The genome of strain G-1-1-1(T)was 6,412,079 bp long with 5176 protein-coding genes. The diagnostic amino acid of cell-wall peptidoglycan of strain G-1-1-1(T)wasmeso-diaminopimelic acid. The only respiratory quinone was menaquinone-9 and the principal polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and unidentified phospholipids. The predominant cellular fatty acids were iso-C-14:0, C-16:1 omega 9c, C-16:0, C(14:0)and anteiso-C-15:0. The DNA G + C content was 61.0 mol%. The anti-SMASH analysis of whole genome showed eight putative biosynthetic gene clusters responsible for various secondary metabolites. Based on genomic, chemotaxonomic, phenotypic and phylogenetic analyses, strain G-1-1-1(T)represents a novel species in the genusLuteobacter, for which the nameLuteolibacter luteussp. nov. is proposed. The type strain is G-1-1-1(T)(= KACC 21614(T) = NBRC 114341(T)).