RETRACTED: Effects of Different Ratio of n-6/n-3 Polyunsaturated Fatty Acids on the PI3K/Akt Pathway in Rats with Reflux Esophagitis (Retracted Article)

Background: We designed this study to investigate the influence of different ratios of n-6/n-3 polyunsaturated fatty acid in the diet of reflux esophagitis (RE) rats' and the effect on the PI3K/Akt pathway. Material/Methods: RE rats were randomly divided into a sham group and modeling groups of different concentrations of n-6/n-3 polyunsaturated fatty acid (PUFA): 12: 1 group, 10: 1 group, 5: 1 group, and 1: 1 group. RT-PCR and Western-blot were used to detect the expression of PI3K, Akt, p-Akt, NF-kappa Bp50, and NF-kappa Bp65 proteins in esophageal tissue. Results: In the n-6/n-3 PUFAs groups the expression of PI3K, Akt, p-Akt, nf-kbp50, and NF-kappa Bp65 mRNA decreased with the decrease in n-6/n-3 ratios in the diet. The lowest expression of each indicator occurred in the 1: 1 n-6/n-3 group compared with other n-6/n-3 groups, the difference was statistically significant (p<0.05). Conclusions: The inhibition of n-3 PUFAs in the development of esophageal inflammation in rats with RE was attributed to the function of PI3K/Akt-NF-kappa B signaling pathway.