PDCD4 suppresses autophagy and promotes apoptosis via Akt in chondrocytes of temporomandibular joint osteoarthritis

被引:3
作者
Liu, Li [1 ]
Feng, Yaping [1 ]
Hu, Shiyu [1 ]
Li, Huimin [1 ]
Li, Yanyan [1 ]
Ke, Jin [1 ]
Long, Xing [2 ]
机构
[1] Wuhan Univ, Sch & Hosp Stomatol, Key Lab Oral Biomed, Minist Educ KLOBM,State Key Lab Breeding Base Bas, Wuhan 430079, Hubei, Peoples R China
[2] Wuhan Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, 237 Luoyu Rd, Wuhan 430079, Hubei, Peoples R China
基金
中国国家自然科学基金;
关键词
Akt; apoptosis; autophagy; chondrocyte; PDCD4; temporomandibular joint osteoarthritis; INTRAARTICULAR INJECTION; CELL-DEATH; EXPRESSION; PATHOGENESIS; GROWTH; DEGENERATION; CYTOPLASM; CARTILAGE; NUCLEUS; KINASE;
D O I
10.1111/odi.13559
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objective This study aimed to investigate the role and mechanisms of programmed cell death 4 (PDCD4) in chondrocytes from temporomandibular joint osteoarthritis (TMJOA) rats. Material and Methods Hematoxylin and eosin, Safranin O, and micro-CT were used to detect the morphologic changes in condyles in rat TMJs receiving iodoacetic acid (MIA). The expression of PDCD4, and autophagy- and apoptosis-related proteins in these condyles was measured by immunohistochemistry. Next, using a small interference RNA against PDCD4 (siPDCD4-1086) and/or specific signaling activators, the alteration of PDCD4, and autophagy- and apoptosis-related proteins, as well as Akt and JNK in the IL-1 beta-treated chondrocytes from rat TMJ, was detected by Western blot, immunofluorescence, and flow cytometry, respectively. Results Elevation of PDCD4 and reduction in Beclin1, LC3II, and Bcl2 were observed in OA-like lesions after MIA injection into rat TMJs. Then, PDCD4 downregulated autophagy and promoted apoptosis in the IL-1 beta-treated chondrocytes. Furthermore, Akt, not JNK, was responsible for PDCD4-induced suppressed autophagy and enhanced apoptosis in the IL-1 beta-treated chondrocytes. Conclusion PDCD4 is highly expressed in the rat cartilage of MIA-induced TMJOA and IL-1 beta-treated primary chondrocytes. PDCD4 inhibits autophagy and promotes apoptosis via Akt in these cells.
引用
收藏
页码:547 / 558
页数:12
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