Localization shift of a sugar transporter contributes to phloem unloading in sweet watermelons

被引：53

作者：

Ren, Yi ^{[1
]}

Sun, Honghe ^{[1
]}

Zong, Mei ^{[1
]}

Guo, Shaogui ^{[1
]}

Ren, Zhijie ^{[2
]}

Zhao, Jianyu ^{[3
]}

Li, Maoying ^{[1
]}

Zhang, Jie ^{[1
]}

Tian, Shouwei ^{[1
]}

Wang, Jinfang ^{[1
]}

Yu, Yongtao ^{[1
]}

Gong, Guoyi ^{[1
]}

Zhang, Haiying ^{[1
]}

He, Hongju ^{[1
]}

Li, Legong ^{[2
]}

Zhang, Xiaolan ^{[3
]}

Liu, Fan ^{[1
]}

Fei, Zhangjun ^{[4
,5
]}

Xu, Yong ^{[1
]}

机构：

[1] Beijing Acad Agr & Forestry Sci, Key Lab Biol & Genet Improvement Hort Crops North, Beijing Key Lab Vegetable Germplasm Improvement, Natl Watermelon & Melon Improvement Ctr, Beijing 100097, Peoples R China

[2] Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China

[3] China Agr Univ, Beijing Key Lab Growth & Dev Regulat Protected Ve, MOE Joint Int Res Lab Crop Mol Breeding, Beijing 100193, Peoples R China

[4] Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA

[5] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA

来源：

NEW PHYTOLOGIST | 2020年 / 227卷 / 06期

基金：

北京市自然科学基金; 中国国家自然科学基金; 美国食品与农业研究所;

关键词：

localization shift; sugar content; sugar phloem unloading; sugar transporter; watermelon; FRUIT; PATHWAY; QTL;

D O I：

10.1111/nph.16659

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Unloading sugar from sink phloem by transporters is complex and much remains to be understood about this phenomenon in the watermelon fruit. Here, we report a novelvacuolar sugar transporter(ClVST1) identified through map-based cloning and association study, whose expression in fruit phloem is associated with accumulation of sucrose (Suc) in watermelon fruit.ClVST1(97)knockout lines show decreased sugar content and total biomass, whereas overexpression ofClVST1(97)increases Suc content. Population genomic and subcellular localization analyses strongly suggest a single-base change at the coding region ofClVST1(97)as a major molecular event during watermelon domestication, which results in the truncation of 45 amino acids and shifts the localization of ClVST1(97)to plasma membranes in sweet watermelons. Molecular, biochemical and phenotypic analyses indicate thatClVST1(97)is a novel sugar transporter for Suc and glucose efflux and unloading. Functional characterization ofClVST1provides a novel strategy to increase sugar sink potency during watermelon domestication.

引用

页码：1858 / 1871

页数：14

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