Potent monoclonal antibodies neutralize Omicron sublineages and other SARS-CoV-2 variants

被引：4

作者：

Chen, Zhaochun ^{[1
]}

Zhang, Peng ^{[2
]}

Matsuoka, Yumiko ^{[3
]}

Tsybovsky, Yaroslav ^{[4
]}

West, Kamille ^{[5
]}

Santos, Celia ^{[3
]}

Boyd, Lisa F. ^{[6
]}

Nguyen, Hanh ^{[1
]}

Pomerenke, Anna ^{[1
]}

Stephens, Tyler ^{[4
]}

Olia, Adam S. ^{[7
]}

Zhang, Baoshan ^{[7
]}

De Giorgi, Valeria ^{[5
]}

Holbrook, Michael R. ^{[8
]}

Gross, Robin ^{[8
]}

Postnikova, Elena ^{[8
]}

Garza, Nicole L. ^{[9
]}

Johnson, Reed F. ^{[9
]}

Margulies, David H. ^{[6
]}

Kwong, Peter D. ^{[7
]}

Alter, Harvey J. ^{[5
]}

Buchholz, Ursula J. ^{[3
]}

Lusso, Paolo ^{[2
]}

Farci, Patrizia ^{[1
]}

机构：

[1] NIAID, Hepat Pathogenesis Sect, Lab Infect Dis, NIH, Bethesda, MD 20892 USA

[2] NIAID, Lab Immunoregulat, NIH, Bethesda, MD USA

[3] NIAID, RNA Viruses Sect, Lab Infect Dis, NIH, Bethesda, MD USA

[4] Leidos Biomed Res Inc, Canc Res Technol Program, Frederick Natl Lab Canc Res, Frederick, MD USA

[5] NIH, Dept Transfus Med, Clin Ctr, Bethesda, MD USA

[6] NIAID, Mol Biol Sect, Lab Immune Syst Biol, NIH, Bethesda, MD USA

[7] NIAID, Vaccine Res Ctr, NIH, Bethesda, MD USA

[8] NIAID, Integrated Res Facil, NIH, Frederick, MD USA

[9] NIAID, SARS CoV 2 Virol Core, Lab Viral Dis, NIH, Bethesda, MD USA

来源：

CELL REPORTS | 2022年 / 41卷 / 05期

基金：

美国国家卫生研究院;

关键词：

RECEPTOR-BINDING DOMAIN; CRYO-EM STRUCTURE; VALIDATION; SPIKE; REPLICATION; INFECTION; LIBRARIES; TOOLS; MODEL;

D O I：

10.1016/j.celrep.2022.111528

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The emergence and global spread of the SARS-CoV-2 Omicron variants, which carry an unprecedented num-ber of mutations, raise serious concerns due to the reduced efficacy of current vaccines and resistance to therapeutic antibodies. Here, we report the generation and characterization of two potent human monoclonal antibodies, NA8 and NE12, against the receptor-binding domain of the SARS-CoV-2 spike protein. NA8 inter-acts with a highly conserved region and has a breadth of neutralization with picomolar potency against the Beta variant and the Omicron BA.1 and BA.2 sublineages and nanomolar potency against BA.2.12.1 and BA.4. Combination of NA8 and NE12 retains potent neutralizing activity against the major SARS-CoV-2 var-iants of concern. Cryo-EM analysis provides the structural basis for the broad and complementary neutral-izing activity of these two antibodies. We confirm the in vivo protective and therapeutic efficacies of NA8 and NE12 in the hamster model. These results show that broad and potent human antibodies can overcome the continuous immune escape of evolving SARS-CoV-2 variants.

引用

页数：25

共 50 条

[1] Antigenicity comparison of SARS-CoV-2 Omicron sublineages with other variants contained multiple mutations in RBD
Li, Qianqian
Zhang, Mengyi
Liang, Ziteng
Zhang, Li
Wu, Xi
Yang, Chaoying
An, Yimeng
Tong, Jincheng
Liu, Shuo
Li, Tao
Cui, Qianqian
Nie, Jianhui
Wu, Jiajing
Huang, Weijin
Wang, Youchun
MEDCOMM, 2022, 3 (02):
[2] Structural and functional characterization of nanobodies that neutralize Omicron variants of SARS-CoV-2
Cornish, Katy
Huo, Jiandong
Jones, Luke
Sharma, Parul
Thrush, Joseph W.
Abdelkarim, Sahar
Kipar, Anja
Ramadurai, Siva
Weckener, Miriam
Mikolajek, Halina
Liu, Sai
Buckle, Imogen
Bentley, Eleanor
Kirby, Adam
Han, Ximeng
Laidlaw, Stephen M.
Hill, Michelle
Eyssen, Lauren
Norman, Chelsea
Le Bas, Audrey
Clarke, John
James, William
Stewart, James P.
Carroll, Miles
Naismith, James H.
Owens, Raymond J.
OPEN BIOLOGY, 2024, 14 (06)
[3] Neutralizing antibodies and their cocktails against SARS-CoV-2 Omicron and other circulating variants
Yang, Yang
Du, Lanying
CELLULAR & MOLECULAR IMMUNOLOGY, 2022, 19 (08) : 962 - 964
[4] Neutralizing antibodies and their cocktails against SARS-CoV-2 Omicron and other circulating variants
Yang Yang
Lanying Du
Cellular & Molecular Immunology, 2022, 19 : 962 - 964
[5] Differences between Omicron SARS-CoV-2 RBD and other variants in their ability to interact with cell receptors and monoclonal antibodies
Giron, Carolina Correa
Laaksonen, Aatto
Barroso da Silva, Fernando Luis
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2023, 41 (12): : 5707 - 5727
[6] Human monoclonal antibodies to the spike subdomain 1 neutralize SARS-CoV-2 and its variants of concern
Bianchini, F.
Crivelli, V.
Abernathy, M. E.
Guerra, C.
Palus, M.
Marcotte, H.
Piralla, A.
Pedotti, M.
De Gasparo, R.
Simonelli, L.
Matkovic, M.
Toscano, C.
Biggiogero, M.
Calvaruso, V.
Rincon, T. Cervantes
Muri, J.
Podesvova, L.
Shanbhag, A.
Celoria, A.
Sgrignani, J.
Stefanik, M.
Honig, V.
Pranclova, V.
Michalcikova, T.
Prochazka, J.
Guerrini, G.
Mehn, D.
Ciabattini, A.
Abolhassani, H.
Jarrossay, D.
Uguccioni, M.
Medaglini, D.
Pan-Hammarstrom, Q.
Calzolai, L.
Baldanti, F.
Franzetti-Pellanda, A.
Garzoni, C.
Sedlacek, R.
Ruzek, D.
Varani, L.
Cavalli, A.
Barnes, C. O.
Robbiani, D. F.
SWISS MEDICAL WEEKLY, 2022, 152 : 10S - 10S
[7] Characterization of SARS-CoV-2 recombinants and emerging Omicron sublineages
Wang, Yuliang
Long, Yiyin
Wang, Feng
Li, Changlin
Liu, Wei
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES, 2023, 20 (01): : 151 - 162
[8] Potent inhibition of diverse Omicron sublineages by SARS-CoV-2 fusion-inhibitory lipopeptides
Zhu, Yuanmei
Hu, Yue
Liu, Nian
Chong, Huihui
He, Yuxian
ANTIVIRAL RESEARCH, 2022, 208
[9] Antibody evasion properties of SARS-CoV-2 Omicron sublineages
Sho Iketani
Lihong Liu
Yicheng Guo
Liyuan Liu
Jasper F.-W. Chan
Yiming Huang
Maple Wang
Yang Luo
Jian Yu
Hin Chu
Kenn K.-H. Chik
Terrence T.-T. Yuen
Michael T. Yin
Magdalena E. Sobieszczyk
Yaoxing Huang
Kwok-Yung Yuen
Harris H. Wang
Zizhang Sheng
David D. Ho
Nature, 2022, 604 : 553 - 556
[10] Antibody evasion properties of SARS-CoV-2 Omicron sublineages
Iketani, Sho
Liu, Lihong
Guo, Yicheng
Liu, Liyuan
Chan, Jasper F-W
Huang, Yiming
Wang, Maple
Luo, Yang
Yu, Jian
Chu, Hin
Chik, Kenn K-H
Yuent, Terrence T-T
Yin, Michael T.
Sobieszczyk, Magdalena E.
Huang, Yaoxing
Yuen, Kwok-Yung
Wang, Harris H.
Sheng, Zizhang
Ho, David D.
NATURE, 2022, 604 (7906) : 553 - +

← 1 2 3 4 5 →