Gdf6 induces commitment of pluripotent mesenchymal C3H10T1/2 cells to the adipocyte lineage

被引：19
作者：
Wang, Shan-Shan [1 ]
Huang, Hai-Yan [1 ,2 ]
Chen, Su-Zhen [1 ]
Li, Xi [1 ,2 ]
Zhang, Wen-Ting [1 ]
Tang, Qi-Qun [1 ,2 ]
机构：
[1] Fudan Univ, Dept Biochem & Mol Biol, Key Lab Mol Med, Minist Educ,Shanghai Med Coll, Shanghai 200032, Peoples R China
[2] Fudan Univ, Inst Stem Cell Res & Regenerat Med, Inst Biomed Sci, Shanghai 200032, Peoples R China
来源：
FEBS JOURNAL | 2013年 / 280卷 / 11期
关键词：
adipogenesis; C3H10T1; 2; commitment; Gdf6; Runx1t1; ACUTE MYELOID-LEUKEMIA; STEM-CELLS; DIFFERENTIATION; IDENTIFICATION; BREAKPOINTS; EXPRESSION; INHIBITOR; BLOCKS; GENE;
D O I：
10.1111/febs.12256
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Mesenchymal stem cells have the potential to undergo commitment and differentiation into a variety of cell types, including osteoblasts, chondrocytes, myocytes and adipocytes. Growth differentiation factor6 (Gdf6) is a member of the transforming growth factor superfamily. We have examined the potential role of Gdf6 in adipogenesis of mesenchymal stem cells, and found that over-expression of Gdf6 induced commitment of pluripotent mesenchymal C3H10T1/2 cells to the adipocyte lineage. The typeI receptor Bmpr1a and the typeII receptors Bmpr2 and Acvr2a mediate the Gdf6 signaling pathway. RNAi silencing of Smad4 and p38 MAPK suggested that both Smad and p38 MAPK pathways are involved in this process. The expression of Runx1t1 was down-regulated in committed pre-adipocytes, and forced expression of Runx1t1 blocked the adipocytic commitment. The results demonstrate a role for Gdf6 in adipocytic commitment and differentiation.
引用
收藏
页码：2644 / 2651
页数：8
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