A new amperometric enzyme electrode for galactose determination

被引：34
|
作者：
Gülce, H [1 ]
Ataman, I
Gülce, A
Yildiz, A
机构：
[1] Suleyman Demirel Univ, Dept Chem, TR-32260 Isparta, Turkey
[2] Hacettepe Univ, Dept Chem, TR-06532 Ankara, Turkey
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 2002年 / 30卷 / 01期
关键词：
D O I：
10.1016/S0141-0229(01)00452-5
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A new enzyme electrode for the determination of galactose was developed by immobilizing galactose oxidase in polyvinylferrocenium matrix coated on a Pt electrode surface. The amperometric response of the enzyme electrode was measured at a constant potential of +0.70V vs SCE. The effects of galactose concentration and temperature on the response of the enzyme electrode were investigated. The response time was found to be 30-40 s and the upper limit of the linear working portion was found to be 40.0 mM galactose concentration. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码：41 / 44
页数：4
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