Critical contribution of linker proteins to Raf kinase activation

被引：51

作者：

Anselmo, AN ^{[1
]}

Bumeister, R ^{[1
]}

Thomas, JM ^{[1
]}

White, MA ^{[1
]}

机构：

[1] Univ Texas, SW Med Ctr, Dept Cell Biol, Dallas, TX 75216 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2002年 / 277卷 / 08期

关键词：

D O I：

10.1074/jbc.M110498200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetic analysis of Ras signaling has unveiled the participation of non-enzymatic accessory proteins in signal transmission. These proteins, KSR, CNK, and Sur-8, can interact with multiple core components of the Ras/MAP kinase cascade and may contribute to the structural organization of this cascade. However, the precise biochemical nature of the contribution of these proteins to Ras signaling is currently unknown. Here we show directly that CNK and KSR are required for stimulus dependent Raf kinase activation. CNK is required for membrane recruitment of Raf, while KSR is likely required to couple Raf to upstream kinases. These results demonstrate that CNK and KSR are integral components of the cellular machinery mediating Raf activation.

引用

页码：5940 / 5943

页数：4

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