Identification of novel recombinants of hepatitis B virus genotypes F and G in human immunodeficiency virus-positive patients from Argentina and Brazil

被引:21
作者
Araujo, Natalia M. [1 ]
Araujo, Oscar C. [1 ]
Silva, Edinete M. [2 ]
Villela-Nogueira, Cristiane A. [3 ]
Nabuco, Leticia C. [3 ]
Parana, Raymundo [4 ]
Bessone, Fernando [5 ]
Gomes, Selma A. [1 ]
Trepo, Christian [2 ]
Kay, Alan [2 ]
机构
[1] Fiocruz MS, Oswaldo Cruz Fdn, Lab Mol Virol, BR-21040360 Rio De Janeiro, Brazil
[2] CNRS, INSERM, U1052, Ctr Rech Cancerol Lyon,UMR 5286, F-69424 Lyon 03, France
[3] Univ Fed Rio de Janeiro, Clementino Fraga Filho Univ Hosp, Hepatol Div, BR-21941970 Rio De Janeiro, Brazil
[4] Univ Fed Bahia, Prof Edgard Santos Univ Hosp, Dept Hepatogastroenterol, BR-40110060 Salvador, BA, Brazil
[5] Univ Nacl Rosario, Sch Med, Dept Gastroenterol & Hepatol, RA-2000 Rosario, Santa Fe, Argentina
关键词
PHYLOGENETIC ANALYSIS; INTERSPECIES RECOMBINATION; MOLECULAR CHARACTERIZATION; NATURAL-HISTORY; E-ANTIGEN; GENOMES; STRAINS; AMPLIFICATION; EPIDEMIOLOGY; COINFECTION;
D O I
10.1099/vir.0.047324-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Hepatitis B virus (HBV) genotype G (HBV/G) infection is almost always detected along with a co-infecting HBV strain that can supply HBeAg, typically HBV/A2. In this study we describe, in two human immunodeficiency virus (HIV)-positive patients from Argentina and Brazil, the first report of HBV/G infection in Argentina and co-circulation of HBV/G, HBV/F and G/F recombinants in the American continent. HBV isolates carrying the 36 bp insertion of HBV/G were the most prevalent in both patients, with >99% of colonies hybridizing to a probe specific for this insertion. Phylogenetic analyses of full-length genomes and precore/core fragments revealed that F4 and F1b were the co-infecting subgenotypes in the Brazilian and Argentinian patients, respectively. Bootscanning analysis provided evidence of recombination in several clones from both patients, with recombination breakpoints located mainly at the precore/core region. These data should encourage further investigations on the clinical implications of HBV/G recombinants in HBV/HIV co-infected patients.
引用
收藏
页码:150 / 158
页数:9
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