Current and Perspective Sensing Methods for Monkeypox Virus

被引：46

作者：

Gul, Ijaz ^{[1
,2
]}

Liu, Changyue ^{[1
,2
]}

Yuan, Xi ^{[1
,2
]}

Du, Zhicheng ^{[1
,2
]}

Zhai, Shiyao ^{[1
,2
]}

Lei, Zhengyang ^{[1
,2
]}

Chen, Qun ^{[1
,2
]}

Raheem, Muhammad Akmal ^{[1
,2
]}

He, Qian ^{[1
,2
]}

Hu, Qiuyue ^{[1
,2
]}

Xiao, Chufan ^{[1
,2
]}

Haihui, Zhang ^{[1
,2
]}

Wang, Runming ^{[1
,2
]}

Han, Sanyang ^{[1
,2
]}

Du, Ke ^{[3
]}

Yu, Dongmei ^{[4
]}

Zhang, Can Yang ^{[1
]}

Qin, Peiwu ^{[1
,2
]}

机构：

[1] Tsinghua Univ, Tsinghua Shenzhen Int Grad Sch, Inst Biopharmaceut & Hlth Engn, Shenzhen 518055, Peoples R China

[2] Tsinghua Univ, Tsinghua Shenzhen Int Grad Sch, Tsinghua Berkeley Shenzhen Inst, Shenzhen 518055, Peoples R China

[3] Univ Calif Riverside, Chem & Environm Engn, Riverside, CA 92521 USA

[4] Shandong Univ, Sch Mech Elect & Informat Engn, Weihai 264209, Peoples R China

来源：

BIOENGINEERING-BASEL | 2022年 / 9卷 / 10期

基金：

中国国家自然科学基金;

关键词：

monkeypox; real-time PCR; LAMP; RPA; immunoassay; diagnosis;

D O I：

10.3390/bioengineering9100571

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The outbreak of the monkeypox virus (MPXV) in non-endemic countries is an emerging global health threat and may have an economic impact if proactive actions are not taken. As shown by the COVID-19 pandemic, rapid, accurate, and cost-effective virus detection techniques play a pivotal role in disease diagnosis and control. Considering the sudden multicountry MPXV outbreak, a critical evaluation of the MPXV detection approaches would be a timely addition to the endeavors in progress for MPXV control and prevention. Herein, we evaluate the current MPXV detection methods, discuss their pros and cons, and provide recommended solutions to the problems. We review the traditional and emerging nucleic acid detection approaches, immunodiagnostics, whole-particle detection, and imaging-based MPXV detection techniques. The insights provided in this article will help researchers to develop novel techniques for the diagnosis of MPXV.

引用

页数：17

共 100 条

[51] Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA [J].