Fluorogenic label to quantify the cytosolic delivery of macromolecules

被引：20
|
作者：
Chao, Tzu-Yuan [1 ]
Raines, Ronald T. [1 ,2 ]
机构：
[1] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA
[2] Univ Wisconsin, Dept Chem, Madison, WI 53706 USA
来源：
MOLECULAR BIOSYSTEMS | 2013年 / 9卷 / 03期
关键词：
BETA-GALACTOSIDASE; RIBONUCLEASE INHIBITOR; FLOW-CYTOMETRY; FLUORESCEIN; VARIANTS; PROTEINS; DESIGN;
D O I：
10.1039/c3mb25552b
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The delivery of a macromolecule to the cytosol of human cells is assessed by using a pendant di-O-glycosylated derivative of fluorescein. Its fluorescence is unmasked by Escherichia coli beta-galactosidase installed in the cytosol. Background is diminished by using RNAi to suppress the expression of GLB1, which encodes a lysosomal beta-galactosidase. This strategy was used to quantify the cytosolic entry of a highly cationic protein, ribonuclease A.
引用
收藏
页码：339 / 342
页数：4
相关论文
共 50 条
  • [31] Cell surface markers of cancer stem cells: diagnostic macromolecules and targets for drug delivery
    Timothy E. Andrews
    Dan Wang
    Daniel A. Harki
    Drug Delivery and Translational Research, 2013, 3 : 121 - 142
  • [32] Optoinjection for efficient targeted delivery of a broad range of compounds and macromolecules into diverse cell types
    Clark, IB
    Hanania, EG
    Stevens, J
    Gallina, M
    Fieck, A
    Brandes, R
    Palsson, BO
    Koller, MR
    JOURNAL OF BIOMEDICAL OPTICS, 2006, 11 (01)
  • [33] Cell surface markers of cancer stem cells: diagnostic macromolecules and targets for drug delivery
    Andrews, Timothy E.
    Wang, Dan
    Harki, Daniel A.
    DRUG DELIVERY AND TRANSLATIONAL RESEARCH, 2013, 3 (02) : 121 - 142
  • [34] Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein for Efficient Gene Editing
    Mout, Rubul
    Ray, Moumita
    Tonga, Gulen Yesilbag
    Lee, Yi-Wei
    Tay, Tristan
    Sasaki, Kanae
    Rotello, Vincent M.
    ACS NANO, 2017, 11 (03) : 2452 - 2458
  • [35] Direct quantification of cytosolic delivery of drug nanocarriers using FlAsH-EDT2
    Rotem, R.
    Prosperi, D.
    Bertolini, J. A.
    Salvioni, L.
    Barbieri, L.
    Rizzuto, M. A.
    Tinelli, V.
    Gori, A.
    Adams, S.
    Colombo, M.
    NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 2023, 47
  • [36] Particle size affects the cytosolic delivery of membranotropic peptide-functionalized platinum nanozymes
    Guarnieri, Daniela
    Melone, Pietro
    Moglianetti, Mauro
    Marotta, Roberto
    Netti, Paolo A.
    Pompa, Pier Paolo
    NANOSCALE, 2017, 9 (31) : 11288 - 11296
  • [37] Mono-guanidine heterolipid based SMEDDS: A promising tool for cytosolic delivery of antineoplastics
    Shete, Harshad
    Sable, Sandip
    Tidke, Pritish
    Selkar, Nilakash
    Pawar, Yogita
    Chakraborty, Avik
    De, Abhijit
    Vanage, Geeta
    Patravale, Vandana
    BIOMATERIALS, 2015, 57 : 116 - 132
  • [38] Engineering gas-foamed large porous particles for efficient local delivery of macromolecules to the lung
    Ungaro, Francesca
    Giovino, Concetta
    Coletta, Ciro
    Sorrentino, Raffaella
    Miro, Agnese
    Quaglia, Fabiana
    EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2010, 41 (01) : 60 - 70
  • [39] Targeted delivery of fluorogenic peptide aptamers into live microalgae by femtosecond laser photoporation at single- cell resolution
    Maeno, Takanori
    Uzawa, Takanori
    Kono, Izumi
    Okano, Kazunori
    Iino, Takanori
    Fukita, Keisuke
    Oshikawa, Yuki
    Ogawa, Taro
    Iwata, Osamu
    Ito, Takuro
    Suzuki, Kengo
    Goda, Keisuke
    Hosokawa, Yoichiroh
    SCIENTIFIC REPORTS, 2018, 8
  • [40] Efficient cytosolic delivery mediated by polymersomes facilely prepared from a degradable, amphiphilic, and amphoteric copolymer
    Huang, Zhonghui
    Teng, Wei
    Liu, Longshan
    Wang, Lichun
    Wang, Qinmei
    Dong, Yugang
    NANOTECHNOLOGY, 2013, 24 (26)
12345