Fluorogenic label to quantify the cytosolic delivery of macromolecules

被引：20
|
作者：
Chao, Tzu-Yuan [1 ]
Raines, Ronald T. [1 ,2 ]
机构：
[1] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA
[2] Univ Wisconsin, Dept Chem, Madison, WI 53706 USA
来源：
MOLECULAR BIOSYSTEMS | 2013年 / 9卷 / 03期
关键词：
BETA-GALACTOSIDASE; RIBONUCLEASE INHIBITOR; FLOW-CYTOMETRY; FLUORESCEIN; VARIANTS; PROTEINS; DESIGN;
D O I：
10.1039/c3mb25552b
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The delivery of a macromolecule to the cytosol of human cells is assessed by using a pendant di-O-glycosylated derivative of fluorescein. Its fluorescence is unmasked by Escherichia coli beta-galactosidase installed in the cytosol. Background is diminished by using RNAi to suppress the expression of GLB1, which encodes a lysosomal beta-galactosidase. This strategy was used to quantify the cytosolic entry of a highly cationic protein, ribonuclease A.
引用
收藏
页码：339 / 342
页数：4
相关论文
共 50 条
  • [1] Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells
    Sharei, Armon
    Trifonova, Radiana
    Jhunjhunwala, Siddharth
    Hartoularos, George C.
    Eyerman, Alexandra T.
    Lytton-Jean, Abigail
    Angin, Mathieu
    Sharma, Siddhartha
    Poceviciute, Roberta
    Mao, Shirley
    Heimann, Megan
    Liu, Sophia
    Talkar, Tanya
    Khan, Omar F.
    Addo, Marylyn
    von Andrian, Ulrich H.
    Anderson, Daniel G.
    Langer, Robert
    Lieberman, Judy
    Jensen, Klavs F.
    PLOS ONE, 2015, 10 (04):
  • [2] Genetically targeted fluorogenic macromolecules for subcellular imaging and cellular perturbation
    Magenau, Andrew J. D.
    Saurabh, Saumya
    Andreko, Susan K.
    Telmer, Cheryl A.
    Schmidt, Brigitte F.
    Waggoner, Alan S.
    Bruchez, Marcel P.
    BIOMATERIALS, 2015, 66 : 1 - 8
  • [3] A Redox-Activatable Fluorescent Sensor for the High-Throughput Quantification of Cytosolic Delivery of Macromolecules
    Wang, Zhaohui
    Luo, Min
    Mao, Chengqiong
    Wei, Qi
    Zhao, Tian
    Li, Yang
    Huang, Gang
    Gao, Jinming
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2017, 56 (05) : 1319 - 1323
  • [4] Cytosolic Delivery of Single-Chain Polymer Nanoparticles
    Hamelmann, Naomi M.
    Paats, Jan-Willem D.
    Paulusse, Jos M. J.
    ACS MACRO LETTERS, 2021, 10 (11) : 1443 - 1449
  • [5] ARMMs as a versatile platform for intracellular delivery of macromolecules
    Wang, Qiyu
    Yu, Jiujiu
    Kadungure, Tatenda
    Beyene, Joseph
    Zhang, Hong
    Lu, Quan
    NATURE COMMUNICATIONS, 2018, 9
  • [6] Disulfide-containing Macromolecules for Therapeutic Delivery
    Jiang, Ziwen
    Thayumanavan, S.
    ISRAEL JOURNAL OF CHEMISTRY, 2020, 60 (1-2) : 132 - 139
  • [7] Diffusion of Macromolecules in the Brain: Implications for Drug Delivery
    Wolak, Daniel J.
    Thorne, Robert G.
    MOLECULAR PHARMACEUTICS, 2013, 10 (05) : 1492 - 1504
  • [8] Polyurea dendrimer for efficient cytosolic siRNA delivery
    Restani, Rita B.
    Conde, Joao
    Baptista, Pedro V.
    Cidade, Maria Teresa
    Braganca, Ana M.
    Morgado, Jorge
    Correia, Ilidio J.
    Aguiar-Ricardo, Ana
    Bonifacio, Vasco D. B.
    RSC ADVANCES, 2014, 4 (97): : 54872 - 54878
  • [9] Bacteria-Based Microdevices for the Oral Delivery of Macromolecules
    Cao, Zhenping
    Lin, Sisi
    Liu, Jinyao
    PHARMACEUTICS, 2021, 13 (10)
  • [10] Fluorination Promotes the Cytosolic Delivery of Genes, Proteins, and Peptides
    Lv, Jia
    Wang, Hui
    Rong, Guangyu
    Cheng, Yiyun
    ACCOUNTS OF CHEMICAL RESEARCH, 2022, 55 (05) : 722 - 733
12345