Quantitative detection of mycobacterial mannophosphoinositides in tuberculosis patients by real-time immuno-PCR assay

被引：3

作者：

Mehta, Promod K. ^{[1
]}

Sharma, Suman ^{[1
,2
]}

Mehta, Neeru ^{[3
]}

Dahiya, Bhawna ^{[1
]}

Singh, Pamela ^{[4
]}

Prashar, Kavita ^{[5
]}

Sheoran, Abhishek ^{[6
]}

Varma-Basil, Mandira ^{[7
]}

Khuller, Gopal K. ^{[8
]}

机构：

[1] Maharshi Dayanand Univ, Ctr Biotechnol, Rohtak 124001, India

[2] Pt BD Sharma Univ Hlth Sci, Dept Microbiol, Rohtak 124001, India

[3] Ambedkar Delhi Skill & Entrepreneurship Univ, Dept Med Elect, Delhi 110092, India

[4] Deenbandhu Chhotu Ram Univ Sci & Technol, Dept Biotechnol, Murthal 131039, India

[5] Dr Babasaheb Ambedkar Marathwada Univ, Badrinarayan Barwale Mahavidyalaya, Jalna 431213, Maharashtra, India

[6] Univ Delhi, Ramanujan Coll, Dept Stat, New Delhi 110019, India

[7] Univ Delhi, Vallabhbhai VB Patel Chest Inst, Microbiol Dept, Delhi 110007, India

[8] Postgrad Inst Med Educ & Res, Dept Biochem, Chandigarh 160012, India

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2022年 / 201卷

关键词：

Diagnosis; ELISA; Mtb; PIMs; Real-time immuno-PCR; TB; CORD FACTOR; DIAGNOSIS;

D O I：

10.1016/j.mimet.2022.106563

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A real-time immuno-PCR assay was deliberated to detect mycobacterial mannophosphoinositides (PIMs). A dynamic range of PIMs (0.9 pg/mL-10 ng/mL) was detected in TB patients, wherein 88.2% and 81.1% sensi-tivities were obtained in pulmonary TB and extrapulmonary TB respectively, with 96-96.4% specificity. This assay may translate into a diagnostic kit.

引用

页数：6

共 27 条

[1]

[Anonymous], 2011, Global Health and Aging

[2] Detection of mycobacterial lipoarabinomannan in serum for diagnosis of active tuberculosis [J].

Brock, Mary ;

Hanlon, David ;

Zhao, Mingwei ;

Pollock, Nira R. .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2020, 96 (02)

[3] GeneXpert on patients with human immunodeficiency virus and smear-negative pulmonary tuberculosis [J].

Cuong, Nguyen Kim ;

Ngoc, Nguyen Bao ;

Hoa, Nguyen Binh ;

Dat, Vu Quoc ;

Nhung, Nguyen Viet .

PLOS ONE, 2021, 16 (07)

[4] Detection of mycobacterial CFP-10 (Rv3874) protein in tuberculosis patients by gold nanoparticle-based real-time immuno-PCR [J].

Dahiya, Bhawna ;

Sharma, Suman ;

Khan, Anish ;

Kamra, Ekta ;

Mor, Preeti ;

Sheoran, Abhishek ;

Sreenivas, Vishnubhatla ;

Varma-Basil, Mandira ;

Gupta, Krishna B. ;

Gupta, Mahesh C. ;

Chaudhary, Dhruva ;

Mehta, Promod K. .

FUTURE MICROBIOLOGY, 2020, 15 (08) :601-612

[5]

Dahiya B, 2019, PATHOG DIS, V77, DOI 10.1093/femspd/ftz049

[6] Rational design of drug-like compounds targeting Mycobacterium marinum MelF protein [J].

Dharra, Renu ;

Talwar, Sakshi ;

Singh, Yogesh ;

Gupta, Rani ;

Cirillo, Jeffrey D. ;

Pandey, Amit K. ;

Kulharia, Mahesh ;

Mehta, Promod K. .

PLOS ONE, 2017, 12 (09)

[7] Clinical Utility of a Commercial LAM-ELISA Assay for TB Diagnosis in HIV-Infected Patients Using Urine and Sputum Samples [J].

Dheda, Keertan ;

Davids, Virginia ;

Lenders, Laura ;

Roberts, Teri ;

Meldau, Richard ;

Ling, Daphne ;

Brunet, Laurence ;

Smit, Richard van Zyl ;

Peter, Jonathan ;

Green, Clare ;

Badri, Motasim ;

Sechi, Leonardo ;

Sharma, Surendra ;

Hoelscher, Michael ;

Dawson, Rodney ;

Whitelaw, Andrew ;

Blackburn, Jonathan ;

Pai, Madhukar ;

Zumla, Alimuddin .

PLOS ONE, 2010, 5 (03)

[8] Systematic Review and Meta-Analysis of Antigen Detection Tests for the Diagnosis of Tuberculosis [J].

Flores, L. L. ;

Steingart, K. R. ;

Dendukuri, N. ;

Schiller, I. ;

Minion, J. ;

Pai, M. ;

Ramsay, A. ;

Henry, M. ;

Laal, S. .

CLINICAL AND VACCINE IMMUNOLOGY, 2011, 18 (10) :1616-1627

[9] The Mycobacterial Cell Envelope-Lipids [J].

Jackson, Mary .

COLD SPRING HARBOR PERSPECTIVES IN MEDICINE, 2014, 4 (10)

[10] Serodiagnosis of tuberculosis:: Comparison of immunoglobulin A (IgA) response to sulfolipid I with IgG and IgM responses to 2,3-diacyltrehalose, 2,3,6-triacyltrehalose, and cord factor antigens [J].

Julián, E ;

Matas, L ;

Pérez, A ;

Alcaide, J ;

Lanéelle, MA ;

Luquin, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (10) :3782-3788

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