The TRPM7 channel is inactivated by PIP2 hydrolysis

被引：416

作者：

Runnels, LW ^{[1
]}

Yue, LX ^{[1
]}

Clapham, DE ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Childrens Hosp, Howard Hughes Med Inst, Boston, MA 02115 USA

来源：

NATURE CELL BIOLOGY | 2002年 / 4卷 / 05期

关键词：

D O I：

10.1038/ncb781

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

TRPM7 (ChaK1, TRP-PLIK, LTRPC7) is a ubiquitous, calcium-permeant ion channel that is unique in being both an ion channel and a serine/threonine kinase. The kinase domain of TRPM7 directly associates with the C2 domain of phospholipase C ( PLC). Here, we show that in native cardiac cells and heterologous expression systems, Galphaq-linked receptors or tyrosine kinase receptors that activate PLC potently inhibit channel activity. Numerous experimental approaches demonstrated that phosphatidylinositol 4,5-bisphosphate (PIP2), the substrate of PLC, is a key regulator of TRPM7. We conclude that receptor-mediated activation of PLC results in the hydrolysis of localized PIP2, leading to inactivation of the TRPM7 channel.

引用

页码：329 / 336

页数：8

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