Label-free dsDNA-Cu NPs-based fluorescent probe for highly sensitive detection of L-histidine

被引：46

作者：

Liu, Ya-Ru ^{[1
]}

Hu, Rong ^{[1
]}

Liu, Tao ^{[1
]}

Zhang, Xiao-Bing ^{[1
]}

Tan, Weihong ^{[1
]}

Shen, Guo-Li ^{[1
]}

Yu, Ru-Qin ^{[1
]}

机构：

[1] Hunan Univ, Mol Sci & Biomed Lab, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn,Coll Biol, Changsha 410082, Hunan, Peoples R China

来源：

TALANTA | 2013年 / 107卷

关键词：

Copper nanoparticles; L-histidine; Label-free; Biosensor; CAPILLARY-ZONE-ELECTROPHORESIS; COPPER NANOPARTICLES; TEMPLATED FORMATION; AMPLIFIED DETECTION; IONS; NANOCLUSTERS;

D O I：

10.1016/j.talanta.2013.01.038

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new and facile strategy using double-stranded DNA-copper nanoparticles (dsDNA-Cu NPs) as fluorescence reporters for the highly sensitive and selective detection of L-histidine was demonstrated. In the dsDNA-Cu NPs-based sensing system, the fluorescence was quenched considerably upon the addition of L-histidine. Under the optimized experimental conditions, the probe exhibits excellent performance (e.g., a satisfactory detection limit of 5 mu M and high specificity). Our in situ method requires no covalent attachment of DNA to a fluorophore, which could significantly reduce the cost and simplify the procedure for L-histidine detection. Moreover, the proposed sensing system could be applicable for the detection of target biomolecule in complex biological samples. These striking properties make it an attractive platform for the direct detection of L-histidine. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：402 / 407

页数：6

共 25 条

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