Development of a Stress-Inducible Controlled Expression (SICE) system in Lactococcus lactis for the production and delivery of therapeutic molecules at mucosal surfaces

被引：82
作者：
Benbouziane, Bouasria [1 ,2 ,3 ]
Ribelles, Pedro [1 ,2 ]
Aubry, Camille [1 ,2 ]
Martin, Rebeca [1 ,2 ]
Kharrat, Pascale [1 ,2 ]
Riazi, Ali [3 ]
Langella, Philippe [1 ,2 ]
Bermudez-Humaran, Luis G. [1 ,2 ]
机构：
[1] INRA, Micalis UMR 1319, Commensal & Probiot Host Interact Lab, F-78350 Jouy En Josas, France
[2] AgroParisTech, Micalis UMR1319, F-78350 Jouy En Josas, France
[3] Univ Mostaganem, Dept Biotechnol, Lab Beneficial Microorganisms Funct Foods & Hlth, Lab Microorganismes Benef Aliments Fonct & Sante, Mostaganem 27000, Algeria
来源：
JOURNAL OF BIOTECHNOLOGY | 2013年 / 168卷 / 02期
关键词：
Lactic acid bacteria; Lactococcus lactis; Immunomodulation; Recombinant heterologous production; Stress-inducible system; HEAT-SHOCK RESPONSE; CONTROLLED GENE-EXPRESSION; ACID BACTERIA; SUBSP LACTIS; INDUCED COLITIS; MURINE COLITIS; E7; ANTIGEN; RECOMBINANT; MICE; PROTEIN;
D O I：
10.1016/j.jbiotec.2013.04.019
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In recent years, recombinant lactic acid bacteria (LAB) have been successfully used as safe mucosal delivery vectors. Herein, we report on the development of a Stress-Inducible Controlled Expression (SICE) system in L. lactis for the production and delivery of proteins of health interest (both therapeutic and vaccine related) at mucosal surfaces. This system is episomal in nature and is composed of a vector carrying an expression cassette under the transcriptional control of a stress-inducible promoter. The functionality of the SICE system was validated in vivo using two different routes of administration: oral and intranasal, and in two different murine models of human pathologies: (i) a model of therapy against inflammatory bowel diseases (IBD) and (ii) a model of vaccination against human papillomavirus type-16 (HPV-16). (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：120 / 129
页数：10
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