Reprogramming the Maternal Zebrafish Genome after Fertilization to Match the Paternal Methylation Pattern

被引:309
作者
Potok, Magdalena E. [1 ,2 ]
Nix, David A. [2 ]
Parnell, Timothy J. [1 ,2 ]
Cairns, Bradley R. [1 ,2 ]
机构
[1] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA
[2] Univ Utah, Sch Med, Huntsman Canc Inst, Dept Oncol Sci, Salt Lake City, UT 84112 USA
关键词
PROMOTER DNA METHYLATION; PREIMPLANTATION DEVELOPMENT; HISTONE MODIFICATIONS; GENE-EXPRESSION; CHROMATIN; EMBRYOGENESIS; EMBRYO; CELLS; DEMETHYLATION; PLURIPOTENT;
D O I
10.1016/j.cell.2013.04.030
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Early vertebrate embryos must achieve totipotency and prepare for zygotic genome activation (ZGA). To understand this process, we determined the DNA methylation (DNAme) profiles of zebrafish gametes, embryos at different stages, and somatic muscle and compared them to gene activity and histone modifications. Sperm chromatin patterns are virtually identical to those at ZGA. Unexpectedly, the DNA of many oocyte genes important for germline functions (i.e., piwil1) or early development (i.e., hox genes) is methylated, but the loci are demethylated during zygotic cleavage stages to precisely the state observed in sperm, even in parthenogenetic embryos lacking a replicating paternal genome. Furthermore, this cohort constitutes the genes and loci that acquire DNAme during development (i.e., ZGA to muscle). Finally, DNA methyltransferase inhibition experiments suggest that DNAnne silences particular gene and chromatin cohorts at ZGA, preventing their precocious expression. Thus, zebrafish achieve a totipotent chromatin state at ZGA through paternal genome competency and maternal genonne DNAme reprogramming.
引用
收藏
页码:759 / 772
页数:14
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