First Report on Callus Induction in Ashoka (Saraca indica): an Important Medicinal Plant

被引：0
作者：
Waman, A. A. [1 ]
Umesha, K. [1 ]
Sathyanarayana, B. N. [1 ]
机构：
[1] Univ Agr Sci Bangalore, GKVK, Dept Hort, Plant Tissue Culture Lab, Bangalore 560065, Karnataka, India
来源：
IV INTERNATIONAL SYMPOSIUM ON ACCLIMATIZATION AND ESTABLISHMENT OF MICROPROPAGATED PLANTS | 2010年 / 865卷
关键词：
leaves; hypocotyls; epicotyls; powdery callus; in vitro; MICROPROPAGATION; REGENERATION; EXPLANTS; L;
D O I：
暂无
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Leaves cultured on Murashige and Skoog (MS) media supplemented with BAP (2 mg/L) and NAA (0.5 mg/L) showed calli formation in 29.4% explants. When cultured on half strength MS media (2 mg/L BAP and 0.5 mg/L NAA), 13.3% explants could produce calli. Hypocotyls segments cultured on full strength MS media showed good callusing response (52.6%). Culture of explants on the half strength MS media produced calli in 77.8% of explants. When segments of epicotyls were placed on full strength MS media, a callusing response of 83.3% was observed. Segments when were placed on half strength MS media, a maximum response of 88.9% was observed. The calli failed to differentiate into shoots when sub-cultured on shoot induction media.
引用
收藏
页码：383 / 386
页数：4
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