Probing the relative orientation of molecules bound to DNA through controlled interference using second-harmonic generation

被引：18

作者：

Doughty, Benjamin ^{[1
]}

Rao, Yi ^{[1
]}

Kazer, Samuel W. ^{[1
]}

Kwok, Sheldon J. J. ^{[1
]}

Turro, Nicholas J. ^{[1
]}

Eisenthal, Kenneth B. ^{[1
]}

机构：

[1] Columbia Univ, Dept Chem, New York, NY 10027 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2013年 / 110卷 / 15期

基金：

美国国家科学基金会;

关键词：

alignment; daunorubicin; equilibrium binding; nonlinear spectroscopy; phase; DAUNOMYCIN; D(CPGPTPAPCPG); RECOGNITION; BINDING;

D O I：

10.1073/pnas.1302554110

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A method is described in which the interference of radiated second-harmonic electric fields generated by a pair of oriented molecules intercalated into double-stranded DNA is controlled and measured. The results show that the relative molecular orientation of the two molecules significantly changes the magnitude of the observed second-harmonic generation intensity, which is described by a simple model that accounts for the interferences of the radiated fields. The technique presented shows promise for future experiments investigating structural changes induced by the formation of a DNA-biomolecule complex.

引用

页码：5756 / 5758

页数：3