Selection of Single Chain Human Monoclonal Antibody (scFv) Against Rabies virus by Phage Display Technology

被引：0
作者：
Pruksametanan, Natcha [1 ]
Yamabhai, Montarop [1 ]
Khawplod, Pakamatz [2 ]
机构：
[1] Suranaree Univ Technol, Phage Display Biotechnol Lab, Sch Biotechnol, Inst Agr Technol, Nakhon Ratchasima, Thailand
[2] Thai Red Cross Soc, Queen Saovabha Mem Inst, Bangkok, Thailand
来源：
6TH IEEE INTERNATIONAL CONFERENCE ON NANO/MOLECULAR MEDICINE AND ENGINEERING (IEEE-NANOMED 2012) | 2012年
关键词：
Phage display; Rabies virus; single-chain fragments(scFv); human monoclonal antibody;
D O I：
暂无
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Human monoclonal antibodies against Rabies virus were selected from non-immunized human scFv library (YAMO-I library) and immunized library (Yamo-Rb library) by using phage display technology. The biopanning was performed for 2-5 rounds by using two types of inactivated rabies vaccines as targets. These are purified vero cell rabies vaccine (PVRV) and purified chick embryo cell vaccine (PCEC). A total of 14 positive clones from various method of biopanning that can bind to rabies, i.e.; IRA7c, IIIRC2c, IRC3c, IYC11c, IYC12c, IYD1c, IYF5c, IIRD5v, IIYB5v, IIYG4v, IIYE5v, IIYG8v, IIYD4v and IVB4cv, were isolated and their genes were sequenced. The ELISA result showed that the positive clones always bind strongly to the targets that were used for biopanning; however some clones can cross-react to the related virus. These selected scFv antibodies will be tested for neutralization activities in vitro in the next step.
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页码：78 / 81
页数：4
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