A method for high-quality RNA extraction from Panax ginseng plant tissues

被引：0

作者：

Wang, K ^{[1
]}

Wang, Y ^{[1
]}

Bao, YL ^{[1
]}

Meng, XY ^{[1
]}

Wu, Y ^{[1
]}

Li, YX ^{[1
]}

机构：

[1] NE Normal Univ, Inst Cytol & Genet, Changchun 130024, Peoples R China

来源：

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 2006年 / 33卷 / 01期

关键词：

RNA extraction; Pnax ginseng; secondary substance;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Extraction of high-quality RNA from Panax ginseng tissues is particularly difficult due to high levels of polyphenolics and polysaccharides, or other compounds that bind and/or coprecipitate with RNA. The methods such as guanidinum thiocyanate, CTAB and modified Trizol were applied to isolate high purity and integrity RNA from Panax ginseng. By all of these methods total RNA can be obtained, but only by the modified Trizol method, it extracts integrity and undegraded RNA from mature leaf tissue. Following this efficient procedure, 90 similar to 120 mu g/g total RNA from leaf tissues can be obtained. The ratio of intensities of the 28 S and 18 S rRNA bands was 2 : 1. The absorbance ratios (A(260)/A(281)) were 1.8 to 2.0. The RNA is suitable for RT-PCR and cDNA library construction.

引用

页码：95 / 99

页数：5

共 16 条

[1]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[2] COLONY BANK CONTAINING SYNTHETIC COL EL HYBRID PLASMIDS REPRESENTATIVE OF ENTIRE ESCHERICHIA-COLI GENOME [J].

CLARKE, L ;

CARBON, J .

CELL, 1976, 9 (01) :91-99

[3] An improved RNA isolation method for succulent plant species rich in polyphenols and polysaccharides [J].

Gehrig, HH ;

Winter, K ;

Cushman, J ;

Borland, A ;

Taybi, T .

PLANT MOLECULAR BIOLOGY REPORTER, 2000, 18 (04) :369-376

[4]

LAKHVIR L, 2001, PLANT MOL BIOL REPT, V19, P181

[5]

LESSARD P, 1997, PLANT MOL BIOL LAB M, P152

[6]

LI H, 1999, BIOTECHNOLOGY INFORM, V15, P36

[7]

LI YF, 2004, J WUHAN BOT RES, V22, P551

[8]

LU Z, 2004, LETT BIOTECHNOLOGY, V15, P372

[9] Isolation of high-quality RNA from white spruce tissue using a three-stage purification method and subsequent cloning of a transcript from the PR-10 gene family [J].

Mattheus, N ;

Ekramoddoullah, AKM ;

Lee, SP .

PHYTOCHEMICAL ANALYSIS, 2003, 14 (04) :209-215

[10] An improved RNA isolation method for plant tissues containing high levels of phenolic compounds or carbohydrates [J].

Salzman, RA ;

Fujita, T ;

Zhu-Salzman, K ;

Hasegawa, PM ;

Bressan, RA .

PLANT MOLECULAR BIOLOGY REPORTER, 1999, 17 (01) :11-17

← 1 2 →