Interlocked feedback loops within the Drosophila circadian oscillator

被引：310

作者：

Glossop, NRJ

Lyons, LC

Hardin, PE ^{[1
]}

机构：

[1] Univ Houston, Dept Biol & Biochem, Houston, TX 77204 USA

[2] Univ Houston, Biol Clocks Program, Houston, TX 77204 USA

来源：

SCIENCE | 1999年 / 286卷 / 5440期

关键词：

D O I：

10.1126/science.286.5440.766

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Drosophila Clock (dClk) is rhythmically expressed, with peaks in mRNA and protein (dCLK) abundance early in the morning. dClk mRNA cycling is shown here to be regulated by PERIOD-TIMELESS (PER-TIM)-mediated release of dCLK- and CYCLE (CYC)-dependent repression. Lack of both PER-TIM derepression and dCLK-CYC repression results in high levels of dClk mRNA, which implies that a separate dClk activator is present. These results demonstrate that the Drosophila circadian feedback loop is composed of two interlocked negative feedback Loops: a per-tim loop, which is activated by dCLK-CYC and repressed by PER-TIM, and a dClk Loop, which is repressed by dCLK-CYC and derepressed by PER-TIM.

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页码：766 / 768

页数：3

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