Lactobacillus plantarum DK119 as a Probiotic Confers Protection against Influenza Virus by Modulating Innate Immunity

被引：132

作者：

Park, Min-Kyung ^{[1
,2
,3
]}

Vu Ngo ^{[2
,3
]}

Kwon, Young-Man ^{[2
,3
]}

Lee, Young-Tae ^{[2
,3
]}

Yoo, Sieun ^{[2
,3
]}

Cho, Young-Hee ^{[4
]}

Hong, Sung-Moon ^{[4
]}

Hwang, Hye Suk ^{[2
,3
]}

Ko, Eun-Ju ^{[2
,3
]}

Jung, Yu-Jin ^{[2
,3
]}

Moon, Dae-Won ^{[5
]}

Jeong, Eun-Ji ^{[5
]}

Kim, Min-Chul ^{[2
,3
,6
]}

Lee, Yu-Na ^{[2
,3
]}

Jang, Ji-Hun ^{[5
]}

Oh, Joon-Suk ^{[5
]}

Kim, Cheol-Hyun ^{[4
]}

Kang, Sang-Moo ^{[2
,3
]}

机构：

[1] Chungwoon Univ, Dept Human Nutr & Food Sci, Hongsung Kun, Chungnam, South Korea

[2] Georgia State Univ, Ctr Inflammat Immun & Infect, Atlanta, GA 30303 USA

[3] Georgia State Univ, Dept Biol, Atlanta, GA USA

[4] Dankook Univ, Dept Anim Resource Sci, Cheonan Si, Chungnam, South Korea

[5] Tobico Inc, Cheonan Si, Chungnam, South Korea

[6] Emory Univ, Sch Med, Dept Microbiol & Immunol, Atlanta, GA 30322 USA

来源：

PLOS ONE | 2013年 / 8卷 / 10期

关键词：

LACTIC-ACID BACTERIA; SSP BULGARICUS OLL1073R-1; IMPROVED CROSS-PROTECTION; CASEI STRAIN SHIROTA; DENDRITIC CELLS; ALVEOLAR MACROPHAGES; HUMAN HEALTH; MICE; INFECTION; RESPONSES;

D O I：

10.1371/journal.pone.0075368

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Lactobacillus plantarum DK119 (DK119) isolated from the fermented Korean cabbage food was used as a probiotic to determine its antiviral effects on influenza virus. DK119 intranasal or oral administration conferred 100% protection against subsequent lethal infection with influenza A viruses, prevented significant weight loss, and lowered lung viral loads in a mouse model. The antiviral protective efficacy was observed in a dose and route dependent manner of DK119 administration. Mice that were treated with DK119 showed high levels of cytokines IL-12 and IFN-gamma in bronchoalveolar lavage fluids, and a low degree of inflammation upon infection with influenza virus. Depletion of alveolar macrophage cells in lungs and bronchoalveolar lavages completely abrogated the DK119-mediated protection. Modulating host innate immunity of dendritic and macrophage cells, and cytokine production pattern appeared to be possible mechanisms by which DK119 exhibited antiviral effects on influenza virus infection. These results indicate that DK119 can be developed as a beneficial antiviral probiotic microorganism.

引用

页数：10

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