Optimal fraction collecting in preparative LC/MS

被引:13
|
作者
Rosentreter, U [1 ]
Huber, U
机构
[1] Bayer HealthCare AG, Pharma Res, D-72096 Wuppertal, Germany
[2] Agilent Technol Deutschland GmbH, D-76337 Waldbronn, Germany
来源
JOURNAL OF COMBINATORIAL CHEMISTRY | 2004年 / 6卷 / 02期
关键词
D O I
10.1021/cc034046a
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
In this paper, we discuss various methods for fraction collection in high-throughput chromatography. UV-triggered fractionation allows precise cutting of peaks. However, valuable fraction collector space is wasted, because many undesired compounds are collected. In mass-triggered fraction collection, the collector space is used more efficiently, because only peaks containing the desired products are collected. Unfortunately, mass peaks are broader than UV peaks, and therefore, fractions contaminated by a closely following peak are often collected. This can be avoided if the collection in preparative LC/MS occurs by a logical AND combination of UV- and mass-triggered collection. The success of this optimal collection mode is shown for three examples.
引用
收藏
页码:159 / 164
页数:6
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