共 23 条
- [21] An Arp2/3 nucleated F-actin shell fragments the nuclear membranes at nuclear envelope breakdown in starfish oocytesMOLECULAR BIOLOGY OF THE CELL, 2013, 24Mori, M.Somogyi, K.Monnier, N.Bathe, M.Lenart, P.
The nuclear F-actin interactome of Xenopus oocytes reveals an actin-bundling kinesin that is essential for meiotic cytokinesis
被引：58
|作者：
Samwer, Matthias
[1
]
Dehne, Heinz-Juergen
[1
]
Spira, Felix
[2
]
Kollmar, Martin
[3
]
Gerlich, Daniel W.
[2
]
Urlaub, Henning
[4
]
Goerlich, Dirk
[1
]
机构：
[1] MPI Biophys Chem, Dept Cellular Logist, D-37077 Gottingen, Germany
[2] Austrian Acad Sci IMBA, Inst Mol Biotechnol, Vienna, Austria
[3] MPI Biophys Chem, Syst Biol Motor Proteins Grp, D-37077 Gottingen, Germany
[4] MPI Biophys Chem, Bioanalyt Mass Spectrometry Grp, D-37077 Gottingen, Germany
来源：
EMBO JOURNAL
|
2013年
/
32卷
/
13期
关键词：
cytokinesis;
kinesins;
meiosis;
nuclear actin;
phalloidin;
MOLECULAR-WEIGHT PROTEIN;
POLAR BODY EMISSION;
BINDING PROTEIN;
RAN;
POLYMERIZATION;
CYTOSKELETON;
LAEVIS;
MUSCLE;
IDENTIFICATION;
LOCALIZATION;
D O I：
10.1038/emboj.2013.108
中图分类号：
Q5 [生物化学];
Q7 [分子生物学];
学科分类号：
071010 ;
081704 ;
摘要：
Nuclei of Xenopus laevis oocytes grow 100 000-fold larger in volume than a typical somatic nucleus and require an unusual intranuclear F-actin scaffold for mechanical stability. We now developed a method for mapping F-actin interactomes and identified a comprehensive set of F-actin binders from the oocyte nuclei. Unexpectedly, the most prominent interactor was a novel kinesin termed NabKin (Nuclear and meiotic actin-bundling Kinesin). NabKin not only binds microtubules but also F-actin structures, such as the intranuclear actin bundles in prophase and the contractile actomyosin ring during cytokinesis. The interaction between NabKin and F-actin is negatively regulated by Importin-beta and is responsive to spatial information provided by RanGTP. Disconnecting NabKin from F-actin during meiosis caused cytokinesis failure and egg polyploidy. We also found actin-bundling activity in Nabkin's somatic paralogue KIF14, which was previously shown to be essential for somatic cell division. Our data are consistent with the notion that NabKin/KIF14 directly link microtubules with F-actin and that such link is essential for cytokinesis.
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页码：1886 / 1902
页数：17
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