Temperature dependence of retention in reversed-phase liquid chromatography on a porous acrylic support

被引：0
|
作者：
Kamiyama, F [1 ]
Yamazaki, K [1 ]
Kawamura, K [1 ]
Kohara, M [1 ]
机构：
[1] SEKISUI FINE CHEM CO LTD,SHIGA,JAPAN
来源：
BIOMEDICAL CHROMATOGRAPHY | 1996年 / 10卷 / 03期
关键词：
STATIONARY PHASES;
D O I：
暂无
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The solute retention mechanism of reversed-phase liquid chromatography is examined by using newly synthesized hydrophilic porous copolymer beads as a stationary phase. It is shown that when water is used as an eluent, the retention time for solutes such as alcohols, ketones and polyethylene glycols increases with temperature. Adsorption behaviour of polyethylene glycol onto the copolymer beads shows that the adsorption also increases with temperature. These observations are explained on the basis of the hypothesis that hydrophobic interaction plays a vital role in solute retention.
引用
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页码：105 / 110
页数：6
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