Neopinone isomerase is involved in codeine and morphine biosynthesis in opium poppy

被引：68
作者：
Dastmalchi, Mehran [1 ,2 ]
Chen, Xue [1 ,2 ]
Hagel, Jillian M. [1 ,2 ]
Chang, Limei [1 ,2 ]
Chen, Rongji [1 ,2 ]
Ramasamy, Sukanya [1 ]
Yeaman, Sam [1 ]
Facchini, Peterj [1 ,2 ]
机构：
[1] Univ Calgary, Dept Biol Sci, Calgary, AB, Canada
[2] Epimeron Inc, Calgary, AB, Canada
来源：
NATURE CHEMICAL BIOLOGY | 2019年 / 15卷 / 04期
关键词：
CONVERSION; PURIFICATION; ALKALOIDS; REDUCTASE; THEBAINE; PROTEINS; PR-10;
D O I：
10.1038/s41589-019-0247-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The isomerization of neopinone to codeinone is a critical step in the biosynthesis of opiate alkaloids in opium poppy. Previously assumed to be spontaneous, the process is in fact catalyzed enzymatically by neopinone isomerase (NISO). Without NISO the primary metabolic products in the plant, in engineered microbes and in vitro are neopine and neomorphine, which are structural isomers of codeine and morphine, respectively. Inclusion of NISO in yeast strains engineered to convert thebaine to natural or semisynthetic opiates dramatically enhances formation of the desired products at the expense of neopine and neomorphine accumulation. Along with thebaine synthase, NISO is the second member of the pathogenesis-related 10 (PR10) protein family recently implicated in the enzymatic catalysis of a presumed spontaneous conversion in morphine biosynthesis.
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页码：384 / +
页数：10
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