Investigation of the role of ansocalcin in the biomineralization in goose eggshell matrix

被引:82
作者
Lakshminarayanan, R [1 ]
Kini, RM [1 ]
Valiyaveettil, S [1 ]
机构
[1] Natl Univ Singapore, Dept Chem, Dept Sci Biol, Singapore 117543, Singapore
关键词
D O I
10.1073/pnas.072658899
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The role of proteins in biomineralization and the mechanism of eggshell formation are not well understood. We have isolated and purified the major protein, ansocalcin from goose eggshell matrix. The amino acid sequence study indicates that ansocalcin is homologous to the chicken eggshell protein, ovocleidin 17, and C-type lectins. Ansocalcin nucleates polycrystalline aggregates of calcite crystals in in vitro mineralization experiments. The polycrystalline aggregates obtained at higher concentration of ansocalcin appears to be similar to the crystals observed at the mamillary layer of the eggshell.
引用
收藏
页码:5155 / 5159
页数:5
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