Development of novel biocompatible thermosensitive anti-adhesive agents using human-derived acellular dermal matrix

被引:10
作者
Jeong, Jong Ju [1 ]
Lee, Dong Won [2 ]
Song, Seung Yong [2 ]
Park, Yerin [3 ]
Kim, Ji Hee [4 ,5 ]
Kim, Jang Il [3 ]
Kim, Hyung Goo [3 ]
Nam, Ki Taek [6 ]
Lee, Won Jai [2 ]
Nam, Kee-Hyun [1 ]
Lee, Ju Hee [4 ,5 ]
机构
[1] Yonsei Univ, Coll Med, Dept Surg, Seoul, South Korea
[2] Yonsei Univ, Coll Med, Inst Human Tissue Restorat, Dept Plast & Reconstruct Surg, Seoul, South Korea
[3] L&C BIO Co Ltd, R&D Ctr, Gyeonggi Do, South Korea
[4] Yonsei Univ, Coll Med, Dept Dermatol, Seoul, South Korea
[5] Yonsei Univ, Coll Med, Cutaneous Biol Res Inst, Seoul, South Korea
[6] Yonsei Univ, Coll Med, Severance Biomed Sci Inst, Seoul, South Korea
来源
PLOS ONE | 2019年 / 14卷 / 02期
关键词
HUMAN PERITONEAL ADHESIONS; POSTOPERATIVE ADHESIONS; THYROIDECTOMY SCARS; IN-VITRO; PREVENTION; BARRIER; MEMBRANE; CHITOSAN; ACID;
D O I
10.1371/journal.pone.0212583
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Postoperative adhesion is a natural phenomenon that occurs in damaged tissue cells. Several anti-adhesion agents are currently used, but there is no leading-edge product with excellent adhesion-preventive effects. The purpose of this study was to develop ideal anti-adhesive agents using human-derived acellular dermal matrix (ADM). We developed 5 new biocompatible thermosensitive anti-adhesion barriers (AABs) using micronized human-derived ADM, hyaluronic acid, and temperature-sensitive and biocompatible synthesized polymers. The biocompatibility, anti-adhesion effect, and biodegradability of these AABs were compared with those of commercial thermosensitive anti-adhesion agents. No cytotoxic effects were observed in vitro and in vivo. Animal testing of adhesion resistance confirmed that the adhesion area, strength, and grade of AAB03 were statistically superior to those of the control group. Factors related to adhesion formation, such as lymphocytes, macrophages, microvessels, and collagen fiber density, were observed using specific staining methods; the results confirmed that AAB03 group exhibited significantly lower macrophage counts, microvessel density, and collagen fiber density than the control groups. Furthermore, AAB03 was completely absorbed by 6 weeks. Thus, AAB03 has the potential to be used as a high-performance anti-adhesion agent.
引用
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页数:14
相关论文
共 34 条
[1]   Adhesion prevention: state of the art [J].
Al-Musawi, D ;
Thompson, JN .
GYNAECOLOGICAL ENDOSCOPY, 2001, 10 (02) :123-130
[2]   Chitosan-PVP-nano silver oxide wound dressing: In vitro and in vivo evaluation [J].
Archana, D. ;
Singh, Brijesh K. ;
Dutta, Joydeep ;
Dutta, P. K. .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2015, 73 :49-57
[3]  
Attard JAP, 2007, CAN J SURG, V50, P291
[4]   Intra-abdominal use of taurolidine or heparin as alternative products to an antiadhesive barrier (Seprafilm®) in adhesion prevention:: An experimental study on mice [J].
Bahadir, Isamettin ;
Oncel, Mustafa ;
Kement, Metin ;
Sahip, Yusuf .
DISEASES OF THE COLON & RECTUM, 2007, 50 (12) :2209-2214
[5]   Macrophage and T-lymphocyte infiltrates in human peritoneal adhesions indicate a chronic inflammatory disease [J].
Binneboesel, Marcel ;
Rosch, Rafael ;
Junge, Karsten ;
Lynen-Jansen, Petra ;
Schumpelick, Volker ;
Klinge, Uwe .
WORLD JOURNAL OF SURGERY, 2008, 32 (02) :296-304
[6]  
BLAUER KL, 1988, FERTIL STERIL, V49, P144
[7]   Peritoneal healing and adhesion formation/reformation [J].
Cheong, YC ;
Laird, SM ;
Li, TC ;
Shelton, JB ;
Ledger, WL ;
Cooke, ID .
HUMAN REPRODUCTION UPDATE, 2001, 7 (06) :556-566
[8]   Effect of polylactic acid film barrier on intra-abdominal adhesion formation [J].
Ersoy, Eren ;
Ozturk, Vedat ;
Yazgan, Aylin ;
Ozdogan, Mehmet ;
Gundogdu, Haldun .
JOURNAL OF SURGICAL RESEARCH, 2008, 147 (01) :148-152
[9]   Effect of oxidized regenerated cellulose (Interceed®) on the expression of tissue plasminogen activator and plasminogen activator inhibitor-1 in human peritoneal fibroblasts and mesothelial cells [J].
Gago, L. April ;
Saed, Ghassan ;
Elhammady, Eslam ;
Diamond, Michael P. .
FERTILITY AND STERILITY, 2006, 86 :1223-1227
[10]   Fewer adhesions induced by laparoscopic surgery? [J].
Gutt, CN ;
Oniu, T ;
Schernmer, P ;
Mehrabi, A ;
Büchler, MW .
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES, 2004, 18 (06) :898-906