Production of isoamyl acetate with immobilized Staphylococcus simulans lipase in a solvent-free system

被引：63

作者：

Ghamgui, H ^{[1
]}

Karra-Chaâbouni, M ^{[1
]}

Bezzine, S ^{[1
]}

Miled, N ^{[1
]}

Gargouri, Y ^{[1
]}

机构：

[1] Lab Biochim & Genie Enzymat Lipases, Tunis 3038, Tunisia

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2006年 / 38卷 / 06期

关键词：

isoamyl acetate; esterification; solvent-free system; immobilized lipase;

D O I：

10.1016/j.enzmictec.2005.08.011

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A non-commercial immobilized lipase preparation from Staphylococcus simulans was used to catalyze the esterification reaction between acetic acid and isoamyl alcohol to synthetize isoamyl acetate (banana favour) in pure substrate conditions (without addition of any organic solvent). The effects of various reaction parameters such as the amount of lipase, the molar ratio of acetic acid as compared to isoamyl alcohol and the amount of initially added water were investigated. The optimum conditions for achieving 64% conversion during 8 It of incubation are 60 IU of immobilized lipase, an acid/alcohol of 2 molar ratio and an initial addition of water of 10% (w/w). No significant loss of the immobilized lipase activity was observed after four cycles of use of the immobilized enzymatic preparation. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：788 / 794

页数：7

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