Rapid and Complete Enzyme Hydrolysis of Lignocellulosic Nanofibrils

被引：28

作者：

Martin-Sampedro, Raquel ^{[1
]}

Filpponen, Ilari ^{[1
]}

Hoeger, Ingrid C. ^{[2
]}

Zhu, J. Y. ^{[3
]}

Laine, Janne ^{[1
]}

Rojas, Orlando J. ^{[1
,2
]}

机构：

[1] Aalto Univ, Dept Forest Prod Technol, Sch Chem Technol, Aalto 00076, Finland

[2] N Carolina State Univ, Dept Forest Biomat, Raleigh, NC 27695 USA

[3] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA

来源：

ACS MACRO LETTERS | 2012年 / 1卷 / 11期

关键词：

QUARTZ-CRYSTAL MICROBALANCE; CELLULOSE FILMS; SURFACE; WOOD;

D O I：

10.1021/mz300484b

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Rapid enzymatic saccharification of lignocellulosic nanofibrils (LCNF) was investigated by monitoring nanoscale changes in mass via quartz crystal microgravimetry and also by measuring reducing sugar yields. In only a few minutes LCNF thin films were completely hydrolyzed upon incubation in multicomponent enzyme systems. Conversion to sugars and oligosaccharides of LCNF dispersed in water occurred in about 4 h (50 degrees C, pH 5). In contrast, a conversion of only 57% was observed for partially crystalline cellulose (Avicel) after 9 h, under same experimental conditions. Under conditions of high enzyme loading the presence of residual lignin and other macromolecules in the cell wall of LCNF did not appear to affect negatively the recorded high hydrolysis rates. Overall, our findings suggest that deconstruction of the cell wall to nanofibrils is an effective pretreatment to facilitate rapid and complete cellulose bioconversion.

引用

页码：1321 / 1325

页数：5

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