Small heat shock protein Hsp27 protects myosin S1 from heat-induced aggregation, but not from thermal denaturation and ATPase inactivation

被引：28

作者：

Markov, Denis I. ^{[1
]}

Pivovarova, Anastasia V. ^{[1
,2
]}

Chernik, Ivan S. ^{[3
]}

Gusev, Nikolai B. ^{[3
]}

Levitsky, Dmitrii I. ^{[1
,4
]}

机构：

[1] Russian Acad Sci, AN Bach Inst Biochem, Moscow 119071, Russia

[2] Moscow MV Lomonosov State Univ, Sch Bioengn & Bioinformat, Moscow, Russia

[3] Moscow MV Lomonosov State Univ, Dept Biochem, Sch Biol, Moscow, Russia

[4] Moscow MV Lomonosov State Univ, AN Belozersky Inst Physicochem Biol, Moscow, Russia

来源：

FEBS LETTERS | 2008年 / 582卷 / 10期

基金：

俄罗斯基础研究基金会;

关键词：

myosin subfragment 1; small heat shock proteins; thermal denaturation; protein aggregation; differential scanning calorimetry; dynamic light scattering;

D O I：

10.1016/j.febslet.2008.03.035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We applied different methods, such as turbidity measurements, dynamic light scattering, differential scanning calorimetry and co-sedimentation assay, to analyze the interaction of small heat shock protein Hsp27 with isolated myosin head ( myosin subfragment 1, S1) under heat-stress conditions. Upon heating at 43 degrees C, Hsp27 effectively suppresses S1 aggregation, and this effect is enhanced by mutations mimicking Hsp27 phosphorylation. However, Hsp27 was unable to prevent thermal unfolding of myosin heads and to maintain their ATPase activity under heat-shock conditions. Structured summary: MINT-6490863, MINT-6490872: LC1 (S1) (uniprotkb: P02602), Myosin subfragment 1 ( S1) ( uniprotkb: P02562) and Hsp27 ( uniprotkb: P04792) physically interact (MI: 0218) by dynamic light scattering ( MI: 0038) MINT-6490833: LC1 ( S1) ( uniprotkb: P02602), Myosin subfragment 1 ( S1) ( uniprotkb: P02562) and Hsp27 ( uniprotkb: P04792) physically interact ( MI: 0218) by cosedimentation ( MI: 0027) MINT-6490770, MINT-6490782: LC1 ( S1) ( uniprotkb: P02602), Myosin subfragment 1 ( S1) ( uniprotkb: P02562) and Hsp27 ( uniprotkb: P04792) physically interact ( MI: 0218) by light scattering ( MI: 0067) (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：1407 / 1412

页数：6

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