Establishment of a Bombyx mori nucleopolyhedrovirus (BmNPV) hyper-sensitive cell line from the silkworm e21 strain

被引：13

作者：

Lee, Jae Man ^{[1
]}

Kawakami, Naoya ^{[1
]}

Mon, Hiroaki ^{[1
]}

Mitsunobu, Hitoshi ^{[1
]}

Iiyama, Kazuhiro ^{[2
]}

Ninaki, Satoshi ^{[3
]}

Maenaka, Katsumi ^{[4
]}

Park, Enoch Y. ^{[5
]}

Kusakabe, Takahiro ^{[1
]}

机构：

[1] Kyushu Univ, Lab Silkworm Sci, Grad Sch Bioresource & Bioenvironm Sci, Higashi Ku, Fukuoka 8128581, Japan

[2] Kyushu Univ, Inst Biol Control, Grad Sch Bioresource & Bioenvironm Sci, Higashi Ku, Fukuoka 8128581, Japan

[3] Tokyo Univ Agr & Technol, Dept Biol Prod, Tokyo 1838538, Japan

[4] Kyushu Univ, Med Inst Bioregulat, Div Struct Biol, Higashi Ku, Fukuoka 8128582, Japan

[5] Shizuoka Univ, Fac Agr, Dept Appl Biol Chem, Shizuoka 4228529, Japan

来源：

BIOTECHNOLOGY LETTERS | 2012年 / 34卷 / 10期

基金：

日本学术振兴会;

关键词：

Baculoviral expression system; Bombyx mori nucleopolyhedrovirus; Hyper-sensitive cell line; Large-scale expression; Luciferase; Enhanced green fluorescent protein; NUCLEAR POLYHEDROSIS-VIRUS; EXPRESSION; BACULOVIRUS; PROTEIN; SYSTEM; SUSCEPTIBILITY; LEPIDOPTERA; BOMBYCIDAE; INFECTION; VECTORS;

D O I：

10.1007/s10529-012-0971-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Baculoviral expression systems, including those of Autographa californica multiple nucleopolyhedrovirus Bombyx mori nucleopolyhedrovirus (BmNPV), are used for recombinant protein production. Four B. mori-derived (BmN4, Bm5, Bmc140, and Bme21) cell lines were infected with recombinant BmNPV viruses expressing firefly luciferase or EGFP as reporters under the control of a viral polyhedrin promoter. Bme21 exhibited significantly higher (100-fold) luciferase activity than BmN4 and Bm5. With the EGFP reporter protein, Bme21 cells showed a marked increase in the ratio of EGFP-positive cells, reaching 90 % on day 4 post-infection, while Bm5 and BmN4 cells had a slow increase in the ratio of their EGFP-positive population. The viral titer in a supernatant of Bme21 cell culture increased faster than those of Bm5 and BmN4 cells. This susceptibility indicates that the Bme21 cell line is useful for large-scale protein expression using BmNPV.

引用

页码：1773 / 1779

页数：7

共 22 条

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