PHNQ fromEvechinus chloroticusSea Urchin Supplemented with Calcium Promotes Mineralization in Saos-2 Human Bone Cell Line

被引：4

作者：

Hou, Yakun ^{[1
]}

Carne, Alan ^{[2
]}

McConnell, Michelle ^{[3
]}

Mros, Sonya ^{[3
]}

Vasileva, Elena A. ^{[4
]}

Mishchenko, Natalia P. ^{[4
]}

Burrow, Keegan ^{[1
]}

Wang, Ke ^{[1
]}

Bekhit, Adnan A. ^{[5
,6
]}

Bekhit, Alaa El-Din A. ^{[1
]}

机构：

[1] Univ Otago, Dept Food Sci, Dunedin 9016, New Zealand

[2] Univ Otago, Dept Biochem, Dunedin 9016, New Zealand

[3] Univ Otago, Dept Microbiol & Immunol, Dunedin 9016, New Zealand

[4] Russian Acad Sci, GB Elyakov Pacific Inst Bioorgan Chem, Far Eastern Branch, Vladivostok 690041, Russia

[5] Alexandria Univ, Fac Pharm, Dept Pharmaceut Chem, Alexandria 21521, Egypt

[6] Univ Bahrain, Coll Hlth Sci, Allied Hlth Dept, Pharm Program, POB 32 038, Sakheer, Bahrain

来源：

MARINE DRUGS | 2020年 / 18卷 / 07期

关键词：

PHNQ; sea urchin; spinochrome; cytotoxicity; Saos-2; cells; mineralized nodule formation; SEA-URCHIN; STRUCTURAL-CHARACTERIZATION; ECHINOCHROME; DIFFERENTIATION; HISTOCHROME; EXTRACTION; PIGMENTS; NAPHTHOQUINONES; OSTEOPOROSIS; MECHANISMS;

D O I：

10.3390/md18070373

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Polyhydroxylated naphthoquinones (PHNQs), known as spinochromes that can be extracted from sea urchins, are bioactive compounds reported to have medicinal properties and antioxidant activity. The MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay showed that pure echinochrome A exhibited a cytotoxic effect on Saos-2 cells in a dose-dependent manner within the test concentration range (15.625-65.5 mu g/mL). The PHNQ extract from New Zealand sea urchinEvechinus chloroticusdid not induce any cytotoxicity within the same concentration range after 21 days of incubation. Adding calcium chloride (CaCl2) with echinochrome A increased the number of viable cells, but when CaCl(2)was added with the PHNQs, cell viability decreased. The effect of PHNQs extracted on mineralized nodule formation in Saos-2 cells was investigated using xylenol orange and von Kossa staining methods. Echinochrome A decreased the mineralized nodule formation significantly (p< 0.05), while nodule formation was not affected in the PHNQ treatment group. A significant (p< 0.05) increase in mineralization was observed in the presence of PHNQs (62.5 mu g/mL) supplemented with 1.5 mM CaCl2. In conclusion, the results indicate that PHNQs have the potential to improve the formation of bone mineral phase in vitro, and future research in an animal model is warranted.

引用

页数：25

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