LncRNA CCTT-mediated RNA-DNA and RNA-protein interactions facilitate the recruitment of CENP-C to centromeric DNA during kinetochore assembly

被引：10

作者：

Zhang, Chong ^{[1
]}

Wang, Dongpeng ^{[2
]}

Hao, Yajing ^{[2
,3
]}

Wu, Shuheng ^{[2
]}

Luo, Jianjun ^{[2
]}

Xue, Yuanchao ^{[2
]}

Wang, Di ^{[2
]}

Li, Guohong ^{[4
]}

Liu, Lihui ^{[2
]}

Shao, Changwei ^{[3
]}

Li, Huiyan ^{[5
]}

Yuan, Jinfeng ^{[5
]}

Zhu, Maoxiang ^{[6
]}

Fu, Xiang-Dong ^{[3
]}

Yang, Xiao ^{[1
]}

Chen, Runsheng ^{[2
]}

Teng, Yan ^{[1
]}

机构：

[1] Beijing Inst Lifeom, Natl Ctr Prot Sci, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 102206, Peoples R China

[2] Chinese Acad Sci, Inst Biophys, CAS Key Lab RNA Biol, Beijing 100101, Peoples R China

[3] Univ Calif San Diego, Inst Genom Med, Dept Cellular & Mol Med, La Jolla, CA 92093 USA

[4] Chinese Acad Sci, CAS Ctr Excellence Biomacromol, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

[5] Natl Ctr Biomed Anal, State Key Lab Prote, Beijing 100039, Peoples R China

[6] Beijing Inst Radiat Med, Beijing Key Lab Radiobiol, Beijing 100850, Peoples R China

来源：

MOLECULAR CELL | 2022年 / 82卷 / 21期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

LONG NONCODING RNA; MOLECULAR-MECHANISMS; READ ALIGNMENT; TRIPLE HELICES; A NUCLEOSOMES; TRANSCRIPTION; RECOGNITION; IDENTITY; CELLS; MAP;

D O I：

10.1016/j.molcel.2022.09.022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Kinetochore assembly on centromeres is central for chromosome segregation, and defects in this process cause mitotic errors and aneuploidy. Besides the well-established protein network, emerging evidence sug-gests the involvement of regulatory RNA in kinetochore assembly; however, it has remained elusive about the identity of such RNA, let alone its mechanism of action in this critical process. Here, we report CCTT, a pre-viously uncharacterized long non-coding RNA (lncRNA) transcribed from the arm of human chromosome 17, which plays a vital role in kinetochore assembly. We show that CCTT highly localizes to all centromeres via the formation of RNA-DNA triplex and specifically interacts with CENP-C to help engage this blueprint protein in centromeres, and consequently, CCTT loss triggers extensive mitotic errors and aneuploidy. These find-ings uncover a non-centromere-derived lncRNA that recruits CENP-C to centromeres and shed critical lights on the function of centromeric DNA sequences as anchor points for kinetochore assembly.

引用

页码：4018 / +

页数：25