Photodissociation and Dissociative Photoionization Mass Spectrometry of Proteins and Noncovalent Protein-Ligand Complexes

被引:42
作者
Canon, Francis [1 ,2 ]
Milosavljevic, Aleksandar R. [4 ]
van der Rest, Guillaume [6 ]
Refregiers, Matthieu [1 ]
Nahon, Laurent [1 ]
Sarni-Manchado, Pascale [5 ]
Cheynier, Veronique [5 ]
Giuliani, Alexandre [1 ,3 ]
机构
[1] Synchrotron SOLEIL, F-91192 Gif Sur Yvette, France
[2] INRA, UMR1324, Ctr Sci Gout & Alimentat, F-21065 Dijon, France
[3] INRA, CEPIA, UAR1008, F-44316 Nantes, France
[4] Univ Belgrade, Inst Phys Belgrade, Belgrade 11080, Serbia
[5] INRA, Sci Oenol UMR1083, F-34060 Montpellier, France
[6] Univ Paris 11, Lab Chim Phys, F-91405 Orsay, France
关键词
dissociative photoionization; mass spectrometry; proteins; tannins; VUV photon activation; PROLINE-RICH PROTEIN; TOP-DOWN PROTEOMICS; SYNCHROTRON-RADIATION; PEPTIDES; TANNINS; BINDING; NM;
D O I
10.1002/anie.201304046
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Weight of evidence: Using synchrotron radiation, photo-fragmentation of an intrinsically disordered protein is probed and compared with classical tandem mass-spectrometry activation techniques. It provides excellent sequence coverage allowing the identification of the protein noncovalent binding sites. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码:8377 / 8381
页数:5
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