Therapeutic effects of bone marrow-derived mesenchymal stem cells on pulmonary impact injury complicated with endotoxemia in rats

被引：29
作者：
Zhao, Yujing [1 ]
Yang, Ce [1 ]
Wang, Haiyan [1 ]
Li, Haisheng [1 ]
Du, Juan [1 ]
Gu, Wei [1 ]
Jiang, Jianxin [1 ]
机构：
[1] Third Mil Med Univ, State Key Lab Trauma Burns & Combined Injury, Inst Surg Res, Daping Hosp, Chongqing 400042, Peoples R China
来源：
INTERNATIONAL IMMUNOPHARMACOLOGY | 2013年 / 15卷 / 02期
关键词：
Acute lung injury; Mesenchymal stem cells; Transplantation; Endotoxemia; Inflammation; RESPIRATORY-DISTRESS-SYNDROME; IN-VIVO; MODEL; EPITHELIUM; THERAPIES; DELIVERY; DEATH;
D O I：
10.1016/j.intimp.2012.12.008
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Previous studies have demonstrated that acute lung injury (ALI) is associated with significant mortality and so far no effective pharmacotherapy is shown to reverse the natural progression. This study aims to investigate whether the transplantation of bone marrow derived mesenchymal stem cells (MSCs) might restore damaged pulmonary function and tissue structure in ALI. By using a sublethal chest impact injury plus endotoxemia model, MSCs were intravenously transplanted into the injured rats 2 h after trauma. The blood samples were obtained for determination of blood gas. Bronchoalveolar lavage fluid (BALF) was collected for analysis of inflammatory cell count and cytokine (TNF-alpha, IL-1 beta, IL-6 and IL-10) levels. The left lower lobes of the lungs, nearby the impact zone, were collected for SRY gene analysis and histological examination and scoring. After engraftment of MSCs, the number of inflammatory cells in BALE was decreased. Meanwhile, the secretions of pro-inflammatory TNF-alpha and IL-6 were alleviated, while the secretion of anti-inflammatory IL-10 was elevated. Engraftment of MSCs improved the pulmonary gas exchanges, alleviated the lung injury, and reduced the rats' mortality. These results suggested that the MSCs based measures might be a promising strategy in trauma and endotoxemia induced ALI treatment. (C) 2012 Elsevier B.V. All rights reserved.
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收藏
页码：246 / 253
页数：8
相关论文
共 40 条
[1]   Repopulation of human pulmonary epithelium by bone marrow cells: A potential means to promote repair [J].
Albera, C ;
Polak, JM ;
Janes, S ;
Griffiths, MJD ;
Alison, MR ;
Wright, NA ;
Navaratnarasah, S ;
Poulsom, R ;
Jeffery, R ;
Fisher, C ;
Burke, M ;
Bishop, AE .
TISSUE ENGINEERING, 2005, 11 (7-8) :1115-1121
[2]   Heparin functionalized PEG gels that modulate protein adsorption for hMSC adhesion and differentiation [J].
Benoit, DSW ;
Anseth, KS .
ACTA BIOMATERIALIA, 2005, 1 (04) :461-470
[3]   Human mesenchymal stem cells suppress chronic airway inflammation in the murine ovalbumin asthma model [J].
Bonfield, Tracey L. ;
Koloze, Mary ;
Lennon, Donald P. ;
Zuchowski, Brandon ;
Yang, Sung Eun ;
Caplan, Arnold I. .
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2010, 299 (06) :L760-L770
[4]   Defining human mesenchymal stem cell efficacy in vivo [J].
Bonfield, Tracey L. ;
Nolan , Mary T. ;
Lennon, Donald P. ;
Caplan, Arnold I. .
JOURNAL OF INFLAMMATION-LONDON, 2010, 7
[5]   The value of positive end-expiratory pressure and FIO2 criteria in the definition of the acute respiratory distress syndrome [J].
Britos, Martin ;
Smoot, Elizabeth ;
Liu, Kathleen D. ;
Thompson, B. Taylor ;
Checkley, William ;
Brower, Roy G. .
CRITICAL CARE MEDICINE, 2011, 39 (09) :2025-2030
[6]   Multipotent mesenchymal stromal cells obtained from diverse human tissues share functional properties and gene-expression profile with CD146+ perivascular cells and fibroblasts [J].
Covas, Dimas T. ;
Panepucci, Rodrigo A. ;
Fontes, Aparecida M. ;
Silva, Wilson A., Jr. ;
Orellana, Maristela D. ;
Freitas, Marcela C. C. ;
Neder, Luciano ;
Santos, Anemari R. D. ;
Peres, Luiz C. ;
Jamur, Maria C. ;
Zago, Marco A. .
EXPERIMENTAL HEMATOLOGY, 2008, 36 (05) :642-654
[7]   Mesenchymal stem cells enhance recovery and repair following ventilator-induced lung injury in the rat [J].
Curley, Gerard F. ;
Hayes, Mairead ;
Ansari, Bilal ;
Shaw, Georgina ;
Ryan, Aideen ;
Barry, Frank ;
O'Brien, Timothy ;
O'Toole, Daniel ;
Laffey, John G. .
THORAX, 2012, 67 (06) :496-501
[8]   Mesenchymal stem cell therapy for the treatment of chronic obstructive pulmonary disease [J].
D'Agostino, Bruno ;
Sullo, Nikol ;
Siniscalco, Dario ;
De Angelis, Antonella ;
Rossi, Francesco .
EXPERT OPINION ON BIOLOGICAL THERAPY, 2010, 10 (05) :681-687
[9]   Exploitation of Herpesvirus Immune Evasion Strategies to Modify the Immunogenicity of Human Mesenchymal Stem Cell Transplants [J].
de la Garza-Rodea, Anabel S. ;
Verweij, Marieke C. ;
Boersma, Hester ;
van der Velde-van Dijke, Ietje ;
de Vries, Antoine A. F. ;
Hoeben, Rob C. ;
van Bekkum, Dirk W. ;
Wiertz, Emmanuel J. H. J. ;
Knaan-Shanzer, Shoshan .
PLOS ONE, 2011, 6 (01)
[10]   Intravenous Administration of 99mTc-HMPAO-Labeled Human Mesenchymal Stem Cells After Stroke: In Vivo Imaging and Biodistribution [J].
Detante, Olivier ;
Moisan, Anaick ;
Dimastromatteo, Julien ;
Richard, Marie-Jeanne ;
Riou, Laurent ;
Grillon, Emmanuelle ;
Barbier, Emmanuel ;
Desruet, Marie-Dominique ;
De Fraipont, Florence ;
Segebarth, Christoph ;
Jaillard, Assia ;
Hommel, Marc ;
Ghezzi, Catherine ;
Remy, Chantal .
CELL TRANSPLANTATION, 2009, 18 (12) :1369-1379
1234