Identification of HIV-1 subtypes and drug resistance mutations among HIV-1-infected individuals residing in Pontianak, Indonesia

被引：8

作者：

Khairunisa, Siti Qamariyah ^{[1
,2
]}

Megasari, Ni Luh Ayu ^{[2
]}

Indriati, Dwi Wahyu ^{[2
]}

Kotaki, Tomohiro ^{[3
]}

Natalia, Diana ^{[4
]}

Nasronudin ^{[2
,5
]}

Kameoka, Masanori ^{[6
]}

机构：

[1] Univ Airlangga, Fac Med, Doctoral Program, Surabaya 60286, Indonesia

[2] Univ Airlangga, Inst Trop Dis, Indonesian Japan Collaborat Res Ctr Emerging & Re, Surabaya 60115, Indonesia

[3] Kobe Univ, Grad Sch Hlth Sci, Dept Publ Hlth, Div Global Infect Dis, Kobe, Hyogo 6540142, Japan

[4] Univ Tanjung Pura, Fac Med, Dept Parasitol, Pontianak 78124, Indonesia

[5] Univ Airlangga, Fac Med, Surabaya 60115, Indonesia

[6] Kobe Univ, Grad Sch Hlth Sci, Dept Publ Hlth, Div Global Infect Dis,Suma Ku, 7-10-2 Tomogaoka, Kobe, Hyogo 6540142, Japan

来源：

GERMS | 2020年 / 10卷 / 03期

关键词：

HIV-1; CRF01_AE; acquired drug resistance; transmitted drug resistance; Indonesia; THERAPY-NAIVE INDIVIDUALS; MOLECULAR EPIDEMIOLOGY; GENOTYPIC CHARACTERIZATION;

D O I：

10.18683/germs.2020.1203

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Introduction The present study investigated the HIV-1 subtype classification in addition to prevalence of drug resistance mutations (DRMs) in antiretroviral therapy (ART)-experienced and ART-naive residents of Pontianak, West Kalimantan, Indonesia. Methods Whole blood samples collected from 30 HIV-1-infected individuals, comprising 19 ART-experienced and 11 ART-naive individuals, were subjected to RNA and DNA extraction, followed by HIV-1 genes amplification and sequencing analysis. HIV-1 subtyping was classified on viral pol genes encoding reverse transcriptase (RT gene) and protease (PR gene) accompanied by the env and gag genes. DRMs in the RT and PR genes were also analyzed. Results CRF01_AE was identified as the predominant circulating recombinant form (CRF) of HIV-1 in both ART-experienced and ART-naive individuals. In addition, CRF02_AG, subtype B, recombinant virus expressing CRF01_AE and subtype B viral genomic fragments, also recombinant virus containing CRF01_AE and CRF02_AG genomic fragments were also identified. Acquired drug resistance (ADR) was identified in 28.5% of ART-experienced individuals, while no transmitted drug resistance was identified in ART-naive individuals. Conclusions This study identified CRF01_AE as the most predominant HIV-1 CRF distributing in Pontianak, Indonesia. The prevalence of ADR is considered to be high; thus, further surveillance is needed in this region.

引用

页码：174 / 183

页数：10

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